Collection Citation: H Davies-Strickleton, James Allsey, George Taylor, Samie Dalgarno, Douglas Dyer, David Knight 2026. Collection of protocols for label-free GAG disaccharide analysis by HILIC-MS/MS of diverse biological tissues. protocols.io https://dx.doi.org/10.17504/protocols.io.x54v956n1l3e/v1
Manuscript citation:
A manuscript of this work is in preparation, which details the method and demonstration of its utility by application to a range of different samples as biological sources.
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: In development
We are still developing and optimizing this collection
Created: November 28, 2025
Last Modified: April 28, 2026
Collection Integer ID: 233746
Keywords: GAG disaccharides, Heparan sulphate, Chondroitin sulphate, Dermatan Sulphate, Hyaluronan, HILIC-MS/MS, gag disaccharide preparation sample preparation of label, gag disaccharide preparation sample preparation, free gag disaccharide analysis by hilic, gag disaccharide analysis, free gag disaccharide analysis, free gag disaccharides from biological tissue, relative quantification of gag disaccharide amount, gag disaccharide preparation, gag disaccharide amount, gag disaccharide standard, free gag disaccharide generation, gag disaccharide, free gag disaccharide, disaccharide composition, disaccharide amount, disaccharide, free glycosaminoglycan, sample preparation of label, ms of diverse biological sample type, biological tissues equimolar stock solution preparation, sample preparation, ms of diverse biological tissue, samples for analysis hilic, equimolar standard preparation, diverse biological sample type, low amounts of diverse biological sample type, total gag amount, standard preparation, ms data acquisition, p
Funders Acknowledgements:
Wellcome Trust Discovery Research Platform (HDS, DD)
Grant ID: 226804/Z/22/Z
Wellcome Trust Career Development Award (DD)
Grant ID: 319823/Z/24/Z
Abstract
This is a collection of detailed step-by-step guides covering label-free Glycosaminoglycan (GAG) disaccharide analysis by HILIC-MS/MS of diverse biological sample types.
Methods are given for sample disruption, label-free GAG disaccharide generation, equimolar standard preparation, sample/standard vialling and HILIC-MS/MS data analysis and processing.
This analysis enables the relative quantification of GAG disaccharide amounts, total GAG amounts and disaccharide composition, from low amounts of diverse biological sample types.
Individual protocols are provided in the collection, and describe:
