Aug 05, 2020
Collection and midgut dissection of mosquito larvae
- 1,domnic colvin
- UM-DAE Centre for Excellence in Basic Sciences
Document Citation: avinash.kale 2020. Collection and midgut dissection of mosquito larvae. protocols.io https://dx.doi.org/10.17504/protocols.io.bjdhki36
License: This is an open access document distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Created: August 05, 2020
Last Modified: August 05, 2020
Document Integer ID: 40073
Abstract
Collection and midgut dissection of mosquito larvae
Collection and midgut dissection of mosquito larvae
The dead Culex quinquefasciatus mosquito larvae in the fourth instar stage were collected from the wild population around the western regions of Mumbai, India. The location coordinates for these collection sites are provided in the Supplementary data 1.The mid-gut of these mosquito larvae was dissected and the gut material was inoculated in nutritionally rich Luria Bertini media. The bacteria obtained were further screened on selective gram-positive media namely, Columbia CN (Colistin, Nalidixic acid) Agar, Phenyl-ethanol Agar(14) and the US Patented media(15).