Jun 22, 2020
Version 4
Cold-leaching extraction. A new methodology for obtaining inhibitory substances produced by bacteria in solid media. V.4
- Catherine Cesa-Luna1,
- Alberto Aguayo-Acosta2,
- Antonino Baez1,
- Jesús Muñoz-Rojas1,
- Verónica Quintero-Hernández3
- 1Ecology and Survival of Microorganisms Group (ESMG), Laboratorio de Ecología Molecular Microbiana (LEMM), Centro de Investigaciones en Ciencias Microbiológicas (CICM), Instituto de Ciencias (IC), Benemérita Universidad Autónoma de Puebla (BUAP), Puebla, Pue., México.;
- 2Department of Microbiology and Immunology, Biological Sciences Faculty, Universidad Autónoma de Nuevo León, Ciudad Universitaria, San Nicolás de la Garza, Nuevo León, México.;
- 3CONACYT – ESMG, LEMM, CICM, IC-BUAP, Puebla, Pue., México.
Protocol Citation: Catherine Cesa-Luna, Alberto Aguayo-Acosta, Antonino Baez, Jesús Muñoz-Rojas, Verónica Quintero-Hernández 2020. Cold-leaching extraction. A new methodology for obtaining inhibitory substances produced by bacteria in solid media.. protocols.io https://dx.doi.org/10.17504/protocols.io.bhs3j6gn
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: June 22, 2020
Last Modified: June 22, 2020
Protocol Integer ID: 38459
Keywords: Antimicrobial extract, Inhibitory substances, Nitrocellulose filter membranes,
Abstract
Inhibitory substances can be obtained and purified by different methodologies. Free cell supernatants obtained by centrifugation and filtration are commonly used for evaluating antimicrobial metabolites produced by bacteria. In this work, a new methodology for obtaining antimicrobial metabolites is proposed, especially implemented for bacteria which are not able to produce these compounds in liquid media; based on the use of nitrocellulose filter membranes and solid culture media.
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