Mar 27, 2026

Coccia et al.

DOI
https://dx.doi.org/10.17504/protocols.io.q26g7njp3lwz/v1
  • 1Icahn School of Medicine at Mount Sinai
Elena Coccia
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DOI: https://dx.doi.org/10.17504/protocols.io.q26g7njp3lwz/v1
Collection Citation: Elena Coccia 2026. Coccia et al.. protocols.io https://dx.doi.org/10.17504/protocols.io.q26g7njp3lwz/v1
License: This is an open access  collection  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: November 06, 2025
Last Modified: March 27, 2026
Collection  Integer ID: 231707
Keywords: ASAPCRN, protocol, coccia et al, first submission
Abstract
PROTOCOLS From Coccia et al. first submission
Files
Protocol
Name
Astrocyte extraction from brain organoids
Version 2
Created by
Image of gustavo.parfitt Parfitt, University College of London
gustavo.parfitt ParfittUniversity College of London
Protocol
Name
Fixation and Immunostaining protocol for 2D cultures
Version 1
Created by
Image of Elena Coccia, Icahn School of Medicine at Mount Sinai
Elena CocciaIcahn School of Medicine at Mount Sinai
Protocol
Name
Western Blot
Version 1
Created by
Image of Elena Coccia, Icahn School of Medicine at Mount Sinai
Elena CocciaIcahn School of Medicine at Mount Sinai
Protocol
Name
Aggregated aSyn Dot Blot assay
Version 1
Created by
Image of gustavo.parfitt Parfitt, University College of London
gustavo.parfitt ParfittUniversity College of London
Protocol
Name
Image-iT™ LIVE Green Caspase Apoptosis Detection
Version 1
Created by
Image of Elena Coccia, Icahn School of Medicine at Mount Sinai
Elena CocciaIcahn School of Medicine at Mount Sinai
Protocol
Name
Lentiviral Production
Version 1
Created by
Image of Camille Goldman, Icahn School of Medicine at Mount Sinai
Camille GoldmanIcahn School of Medicine at Mount Sinai
Protocol
Name
Astrocyte Viral Transduction
Version 1
Created by
Image of Camille Goldman, Icahn School of Medicine at Mount Sinai
Camille GoldmanIcahn School of Medicine at Mount Sinai
Protocol
Name
qPCR
Version 1
Created by
Image of Camille Goldman, Icahn School of Medicine at Mount Sinai
Camille GoldmanIcahn School of Medicine at Mount Sinai
Protocol
Name
Lysosome proteolysis analysis with DQ-BSA
Version 1
Created by
Image of gustavo.parfitt Parfitt, University College of London
gustavo.parfitt ParfittUniversity College of London
Protocol
Name
Midbrain organoid differentiation in spinner flasks.
Version 1
Created by
Image of gustavo.parfitt Parfitt, University College of London
gustavo.parfitt ParfittUniversity College of London
Protocol
Name
Human Induced Pluripotent Stem Cell (hiPSC) Culture and Passaging Protocol
Version 1
Created by
Image of Elena Coccia, Icahn School of Medicine at Mount Sinai
Elena CocciaIcahn School of Medicine at Mount Sinai
Protocol
Name
Differentiation of iPSCs into NGN2 Neurons Protocol
Version 1
Created by
Image of Elena Coccia, Icahn School of Medicine at Mount Sinai
Elena CocciaIcahn School of Medicine at Mount Sinai
Protocol
Name
Protocol for Dissociation and Culture of Midbrain Astrocytes and Neurons
Version 1
Created by
Image of Elena Coccia, Icahn School of Medicine at Mount Sinai
Elena CocciaIcahn School of Medicine at Mount Sinai
Protocol
Name
Astrocyte-Conditioned Media (ACM) cultures
Version 1
Created by
Image of Elena Coccia, Icahn School of Medicine at Mount Sinai
Elena CocciaIcahn School of Medicine at Mount Sinai
Protocol
Name
Protocol for Live-Cell Imaging of Astrocytes Exposed to HiLyte™ 488-labeled Human Recombinant α-Synuclein
Version 1
Created by
Image of Elena Coccia, Icahn School of Medicine at Mount Sinai
Elena CocciaIcahn School of Medicine at Mount Sinai
Protocol
Name
Quantification of Cell Death and Cytotoxicity Using LDH-Glo™ Cytotoxicity Assay
Version 1
Created by
Image of Elena Coccia, Icahn School of Medicine at Mount Sinai
Elena CocciaIcahn School of Medicine at Mount Sinai
Protocol
Name
Evaluation of the Inflammatory Secretome of Astrocytes Using Cytokine Array
Version 1
Created by
Image of Elena Coccia, Icahn School of Medicine at Mount Sinai
Elena CocciaIcahn School of Medicine at Mount Sinai