Oct 18, 2021

Public workspaceCoBG-11 preparation

DOI
dx.doi.org/10.17504/protocols.io.by6hpzb6
  • 1indian ins
  • iGEM IISER Pune India 2021
Arshshaikh
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DOI: dx.doi.org/10.17504/protocols.io.by6hpzb6
External link: https://link.springer.com/article/10.1186/s13068-020-01720-0#Sec9
Protocol Citation: Arshshaikh 2021. CoBG-11 preparation. protocols.io https://dx.doi.org/10.17504/protocols.io.by6hpzb6
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it’s working
Created: October 18, 2021
Last Modified: October 18, 2021
Protocol Integer ID: 54185
Keywords: Cyanobacteria, E. coli, coculture, growth media
Abstract
CoBG-11 is a coculture medium used to coculture cyanobacteria and E. coli by Zhang et al (2020). It is optimized for E. coli growth. Here are its components:

1. 150 mM NaCl,
2. 4 mM NH4Cl
3. 3 g/L 2-[[1,3-dihydroxy-2-(hydroxymethyl) propan-2-yl] amino] ethanesulfonic acid (TES)

The pH value is adjusted with NaOH to 8.3.

The protocol below describes the preparation of 100ml of coBG-11.
Measure Amount0.88 g NaCl , Amount0.0214 g NH4Cl , and Amount0.3 g TES buffer and add it to a 150mL flask.
Note
These calculations have been made for 100 mL coBG-11. For further information check the reference.

CITATION
Zhang L, Chen L, Diao J, Song X, Shi M, Zhang W (2020). Construction and analysis of an artificial consortium based on the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973 to produce the platform chemical 3-hydroxypropionic acid from CO2.. Biotechnology for biofuels.
LINK
https://doi.org/10.1186/s13068-020-01720-0

Add Amount50 mL BG-11 to the flask and mix till contents dissolve. If salt persists, add 10mL more of BG-11 and mix well.

Once contents dissolve, add BG-11 up to 95 mL.
Adjust pH of the solution to 8.3 with NaOH.
Fill BG-11 up to 100 mL measure pH and adjust if required.
Note
Autoclave CoBG-11 before using.

Citations
Step 1
Zhang L, Chen L, Diao J, Song X, Shi M, Zhang W. Construction and analysis of an artificial consortium based on the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973 to produce the platform chemical 3-hydroxypropionic acid from CO2.
https://doi.org/10.1186/s13068-020-01720-0