Sep 03, 2025

Public workspaceCo-pathology Manuscript Protocols 2025

  • Jhodi Webster1,
  • Asta Zane1,
  • Vickie Yang1,
  • Aidan Miller1,
  • Ashley Harms1
  • 1University of Alabama at Birmingham
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Collection CitationJhodi Webster, Asta Zane, Vickie Yang, Aidan Miller, Ashley Harms 2025. Co-pathology Manuscript Protocols 2025. protocols.io https://dx.doi.org/10.17504/protocols.io.j8nlky8nxg5r/v1
Manuscript citation:
Webster, J. M., et al. (2024). "Amyloid-beta, alpha-synuclein and tau aggregated co-pathologies enhance neuropathology and neuroinflammation." bioRxiv: 2024.2010.2013.618101.

License: This is an open access collection distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: August 26, 2025
Last Modified: September 03, 2025
Collection Integer ID: 225525
Keywords: ASAPCRN, neuropathology in vivo, neuropathology, neuroinflammation, flow cytometry, immunohistochemistry, methods for immunohistochemistry, immunofluorescence
Funders Acknowledgements:
Aligning Science Across Parkinson's
Grant ID: ASUB00000984
Abstract
A collection of protocols included in the study assessing the role of co-pathologies on neuroinflammation and neuropathology in vivo. This collection includes optimized methods for immunohistochemistry (IHC), immunofluorescence (IF), flow cytometry, behavior techniques and quantification methods (stereology for example) developed by members of the Harms Lab and collaborators.
Troubleshooting
Files
Protocol
Name
Mouse Stereotaxic Surgeries
Version 1
, University of Alabama at Birmingham
Jhodi WebsterUniversity of Alabama at Birmingham
Protocol
Name
Brain Single Cell Isolation for Flow Cytometry
Version 1
, University of Alabama at Birmingham
Jhodi WebsterUniversity of Alabama at Birmingham
Protocol
Name
Free Floating DAB Staining
Version 1
, University of Alabama at Birmingham
Jhodi WebsterUniversity of Alabama at Birmingham
Protocol
Name
Free Floating IHC Fluorescent Staining
Version 1
, University of Alabama at Birmingham
Jhodi WebsterUniversity of Alabama at Birmingham
Protocol
Name
Barnes Maze
Version 1
, University of Alabama at Birmingham
Jhodi WebsterUniversity of Alabama at Birmingham
Protocol
Name
Pole Test
Version 1
, University of Alabama at Birmingham
Jhodi WebsterUniversity of Alabama at Birmingham
Protocol
Name
Unbiased Stereology (MBF Bioscience, StereoInvestigator Software)
Version 1
, University of Alabama at Birmingham
Jhodi WebsterUniversity of Alabama at Birmingham
Protocol
Name
Mouse Perfusions
Version 1
, University of Alabama at Birmingham
Jhodi WebsterUniversity of Alabama at Birmingham
Protocol
Name
Cryoprotection of Mouse Brain
Version 1
, University of Alabama at Birmingham
Jhodi WebsterUniversity of Alabama at Birmingham
Protocol
Name
Serial Sectioning of Mouse Brain
Version 1
, University of Alabama at Birmingham
Jhodi WebsterUniversity of Alabama at Birmingham
Protocol
Name
Rotarod Test
Version 1
, University of Alabama at Birmingham
Jhodi WebsterUniversity of Alabama at Birmingham
Protocol
Name
TRT/SDS Sol/Insol Fractionation for Lysate prep from Mouse Brains
Version 1
, University of Alabama at Birmingham
Jhodi WebsterUniversity of Alabama at Birmingham
Protocol
Name
Western Blot
Version 1
, University of Alabama at Birmingham
Jhodi WebsterUniversity of Alabama at Birmingham
Protocol
Name
Open Field
Version 1
, University of Alabama at Birmingham
Ashley AdamsonUniversity of Alabama at Birmingham
Protocol
Icon representing the file  Sonication of α-synuclein Fibrils for injection into the mouse brain
Name
Fork Sonication of α-synuclein Fibrils for injection into the mouse brain
Version 3
, Yale University
Saroj Kumar SahYale University