Apr 23, 2026

Chen C, et al, 2025, doi: 10.1101/2025.06.23.661126.

  • 1National Human Genome Research Institute
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Collection CitationYu Chen 2026. Chen C, et al, 2025, doi: 10.1101/2025.06.23.661126.. protocols.io https://dx.doi.org/10.17504/protocols.io.bp2l6zy2dgqe/v1
License: This is an open access  collection  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: October 16, 2025
Last Modified: April 23, 2026
Collection  Integer ID: 230000
Keywords: ASAPCRN, dopaminergic neuron, gba1 isogenic, gaucher disease, parkinsonism, gba1
Funders Acknowledgements:
ASAP
Grant ID: ASAP-024297
Abstract
This collection provides detailed methods used in our manuscript "Bidirectional regulation of glycoprotein nonmetastatic melanoma protein B by β-glucocerebrosidase deficiency in GBA1 isogenic dopaminergic neurons from a patient with Gaucher disease and parkinsonism". bioRxiv 2025.06.23.661126; doi: https://doi.org/10.1101/2025.06.23.661126
Files
Protocol
Name
LysoIP from iPSC-derived mDANs
Version 1
Created by
Image of Yu Chen, National Human Genome Research Institute
Yu ChenNational Human Genome Research Institute
Protocol
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In-vitro GCase Activity Assay
Version 1
Created by
Image of Yu Chen, National Human Genome Research Institute
Yu ChenNational Human Genome Research Institute
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Ventral midbrain dopaminergic (mDA) Neuron Monolayer Differentiation Protocol
Version 1
Created by
Image of Yu Chen, National Human Genome Research Institute
Yu ChenNational Human Genome Research Institute
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iPSC karyotyping
Version 1
Created by
Image of Yu Chen, National Human Genome Research Institute
Yu ChenNational Human Genome Research Institute
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Endo H/ PNGase F Assay for JESS Automated Western Blot
Version 1
Created by
Image of Yu Chen, National Human Genome Research Institute
Yu ChenNational Human Genome Research Institute
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GBA1 HDR Donor Vector Assembly
Version 1
Created by
Image of Yu Chen, National Human Genome Research Institute
Yu ChenNational Human Genome Research Institute
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Targeted PacBio HiFi Long-Read Amplicon Sequencing GBA1 and GBAP
Version 1
Created by
Image of Yu Chen, National Human Genome Research Institute
Yu ChenNational Human Genome Research Institute
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Flow cytometry to quantify tdTomato+ mDANs
Version 1
Created by
Image of Yu Chen, National Human Genome Research Institute
Yu ChenNational Human Genome Research Institute
Protocol
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Immunocytochemistry with mDANs
Version 1
Created by
Image of Yu Chen, National Human Genome Research Institute
Yu ChenNational Human Genome Research Institute