Apr 15, 2019
Centrifuge the whole blood to separate red blood cells
- 1University of Connecticut
Protocol Citation: George Lykotrafitis 2019. Centrifuge the whole blood to separate red blood cells. protocols.io https://dx.doi.org/10.17504/protocols.io.zzwf77e
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol in our group and it is working.
Created: April 11, 2019
Last Modified: April 15, 2019
Protocol Integer ID: 22294
Abstract
Centrifuge the whole blood in order to obtain the red blood cells.
Materials
Human whole blood, centrifuge, centrifuge tubes.
Centrifuge the whole blood using the parameters: 500 ×g, 4 ℃, and 5 min.
Remove the plasma and buffy coat.
Add the same volume of Alsever's solution as the remaining blood components.
Centrifuge again using the parameters: 500 ×g, 4 ℃, and 5 min.
Remove the top layer.
Add the same volume of Alsever's solution as the remaining blood components.
Centrifuge a third time using the parameters: 500 ×g, 4 ℃, and 5 min.
Remove the top layer.
Add the same volume of Alsever's solution as the remaining blood component - red blood cells, and store at 4 ℃ for up to 7 days.