Mar 05, 2026
Cellular morphology assay of M231 by Giemsa staining
- Guangli Suo1
- 1CAS Key Laboratory of Nano-Bio Interface, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences
- MTS
Protocol Citation: Guangli Suo 2026. Cellular morphology assay of M231 by Giemsa staining. protocols.io https://dx.doi.org/10.17504/protocols.io.kqdg3drzql25/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: March 05, 2026
Last Modified: March 05, 2026
Protocol Integer ID: 262568
Keywords: cellular morphology assay of m231, m231 cells in monolayer culture, cellular morphology of m231, m231 cell, cellular morphology assay, phase contrast microscope, cellular morphology, staining assay, cell, cytoplasm, m231, monolayer culture, giemsa dye
Abstract
The cellular morphology of M231 and MTS-M231 cells in monolayer culture was assessed using the Giemsa staining assay. Briefly, a total of 50,000 cells were seeded in each well of a 6-well plate and cultured for 3 days in complete medium. Subsequently, the cells were fixed with 70% ethanol for 10 minutes and stained with Giemsa dye (Beyotime) for 30 minutes. The nuclei were stained with a purplish-red color, while the cytoplasm exhibited a blue hue. The images were captured using a phase contrast microscope (Scope A1, Zeiss).
Troubleshooting
Cellular morphology assay of M231 by Giemsa staining
Seed a total of 50,000 cells in each well of a 6-well plate and culture for 3 days in complete medium.
Fix the cells with 70% ethanol for 10 minutes.
Stain the cells with Giemsa dye (Beyotime) for 30 minutes.
Observe that the nuclei are stained with a purplish-red color, while the cytoplasm exhibits a blue hue.
Capture images using a phase contrast microscope (Scope A1, Zeiss).