Sep 23, 2025

Public workspaceCell-Titer Glo for iPSC derived neurons

DOI
https://dx.doi.org/10.17504/protocols.io.bp2l6z35kgqe/v1
  • Ellen Hertz1,
  • Yu Chen1,
  • Gani Perez1,
  • Ellen Sidransky1
  • 1National Institutes of Health
Ellen Hertz
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DOI: https://dx.doi.org/10.17504/protocols.io.bp2l6z35kgqe/v1
Protocol Citation: Ellen Hertz, Yu Chen, Gani Perez, Ellen Sidransky 2025. Cell-Titer Glo for iPSC derived neurons. protocols.io https://dx.doi.org/10.17504/protocols.io.bp2l6z35kgqe/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: September 23, 2025
Last Modified: September 23, 2025
Protocol Integer ID: 228000
Keywords: ASAPCRN, titer glo for ipsc, titer glo viability assay, derived neurons cell, derived neuron, neuronal precursor, neurons cell, titer glo, ipsc, cell
Funders Acknowledgements:
ASAP
Grant ID: ASAP-000458
Abstract
Cell-Titer Glo viability assay for iPSC derived neurons and neuronal precursors
Protocol materials
ReagentCellTiter-Glo® Luminescent Cell Viability AssayPromegaCatalog #G7570
Troubleshooting
Cell culture
Seed cells in Amount200 µL media in white opaque 96-wells with clear bottom after regular dissociation according to Kim et al. on day 11 or day 25 respectively.
Outer wells of plate are filled with water to avoid evaporation in measurement wells and one row with media for background measurements.
Change media ( Amount180 µL )with geneticin additive in ascending doses daily for neuronal precursors and every 3 days for neurons

Cell Glo
On DIV 19 or 36 respectively; ReagentCellTiter-Glo® Luminescent Cell Viability AssayPromegaCatalog #G7570 is resuspended with buffer in kit according to instructions

Take of Amount100 µL of media and add Amount100 µL of Cell-Glo reagent (1:1 vol/vol)

Place on orbital shaker for 2 minutes atTemperatureRoom temperature

Incubate in the dark for 10 min at TemperatureRoom temperature

Place plate in plate reader; read luminescence (bottom read, integration 1500 ms)