May 20, 2026

Cascalho Sati et al_Spermidine suppresses glial inflammation and parkinsonian abnormalities in ATP13A2 deficiency

DOI
https://dx.doi.org/10.17504/protocols.io.e6nvww45wvmk/v1
  • 1KU Leuven
Aisha Sati
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DOI: https://dx.doi.org/10.17504/protocols.io.e6nvww45wvmk/v1
Collection Citation: Aisha Sati 2026. Cascalho Sati et al_Spermidine suppresses glial inflammation and parkinsonian abnormalities in ATP13A2 deficiency. protocols.io https://dx.doi.org/10.17504/protocols.io.e6nvww45wvmk/v1
License: This is an open access  collection  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: March 17, 2026
Last Modified: May 20, 2026
Collection  Integer ID: 313438
Keywords: ASAPCRN, spermidine suppresses glial inflammation, parkinsonian abnormalities in atp13a2 deficiency, spermidine suppress, cascalho sati et al-spermidine, atp13a2 deficiency, glial inflammation, spermidine, parkinsonian abnormality, deficiency
Funders Acknowledgements:
Aligning Science Across Parkinson's
Grant ID: ASAP-024297
Abstract
A collection of protocols used for the generation of all data incorporated into the manuscript titled "Spermidine suppresses glial inflammation and parkinsonian abnormalities in ATP13A2 deficiency"
Files
Protocol
Name
Polyamine modulation through IP injections
Version 1
Created by
Image of Hanne HD Dhondt, KU Leuven
Hanne HD DhondtKU Leuven
Protocol
Name
Polyamine modulation in drinking water
Version 1
Created by
Image of Hanne HD Dhondt, KU Leuven
Hanne HD DhondtKU Leuven
Protocol
Name
Metabolomics for measuring polyamine levels in the plasma, brain and liver of mice
Version 1
Created by
Image of Hanne HD Dhondt, KU Leuven
Hanne HD DhondtKU Leuven
Protocol
Name
Open field protocol
Version 1
Created by
Image of Hanne HD Dhondt, KU Leuven
Hanne HD DhondtKU Leuven
Protocol
Name
Pole test protocol
Version 1
Created by
Image of Hanne HD Dhondt, KU Leuven
Hanne HD DhondtKU Leuven
Protocol
Name
Buried pellet test protocol
Version 1
Created by
Image of Hanne HD Dhondt, KU Leuven
Hanne HD DhondtKU Leuven
Protocol
Name
GI function test
Version 1
Created by
Image of Hanne HD Dhondt, KU Leuven
Hanne HD DhondtKU Leuven
Protocol
Name
Limb clasping protocol
Version 1
Created by
Image of Hanne HD Dhondt, KU Leuven
Hanne HD DhondtKU Leuven
Protocol
Name
Perfusion of mice
Version 1
Created by
Image of Hanne HD Dhondt, KU Leuven
Hanne HD DhondtKU Leuven
Protocol
Name
Brain dissection protocol
Version 1
Created by
Image of Hanne HD Dhondt, KU Leuven
Hanne HD DhondtKU Leuven
Protocol
Name
Brain tissue agarose embedding for generating sagittal sections
Version 1
Created by
Image of Hanne HD Dhondt, KU Leuven
Hanne HD DhondtKU Leuven
Protocol
Name
Facial vein blood collection
Version 1
Created by
Image of Hanne HD Dhondt, KU Leuven
Hanne HD DhondtKU Leuven
Protocol
Name
IHC staining (DAB) of Iba1 and GFAP
Version 1
Created by
Image of Hanne HD Dhondt, KU Leuven
Hanne HD DhondtKU Leuven
Protocol
Name
IF staining CD68
Version 1
Created by
Image of Hanne HD Dhondt, KU Leuven
Hanne HD DhondtKU Leuven
Protocol
Name
Atp13a2 Flox and Atp13a2 Null genotyping
Version 1
Created by
Image of Hanne HD Dhondt, KU Leuven
Hanne HD DhondtKU Leuven
Protocol
Name
Microglia isolation using magnetic cell sorting from adult mouse brains
Version 1
Created by
Image of Hanne HD Dhondt, KU Leuven
Hanne HD DhondtKU Leuven
Protocol
Name
MSD Proinflammatory cytokine Panel 1 (mouse) Kit
Version 1
Created by
Image of Hanne HD Dhondt, KU Leuven
Hanne HD DhondtKU Leuven
Protocol
Name
Collection of mouse cerebrospinal fluid from the cisterna magna
Version 1
Created by
Image of Hanne HD Dhondt, KU Leuven
Hanne HD DhondtKU Leuven
Protocol
Name
Primary murine microglia, neurons and co-cultures
Version 1
Created by
Image of Hanne HD Dhondt, KU Leuven
Hanne HD DhondtKU Leuven
Protocol
Name
Fixation and Immunostaining protocol for 2D cultures
Version 1
Created by
Image of Elena Coccia, Icahn School of Medicine at Mount Sinai
Elena CocciaIcahn School of Medicine at Mount Sinai
Protocol
Name
Human Induced Pluripotent Stem Cell (hiPSC) Culture and Passaging Protocol
Version 1
Created by
Image of Elena Coccia, Icahn School of Medicine at Mount Sinai
Elena CocciaIcahn School of Medicine at Mount Sinai
Protocol
Name
Induced Microglia differentiation from iPSC
Version 1
Created by
Image of Elena Coccia, Icahn School of Medicine at Mount Sinai
Elena CocciaIcahn School of Medicine at Mount Sinai
Protocol
Name
CellROX Green Live Imaging Protocol
Version 1
Created by
Image of Elena Coccia, Icahn School of Medicine at Mount Sinai
Elena CocciaIcahn School of Medicine at Mount Sinai
Protocol
Name
Polyamine Pathway Modulation in 2D Monoculture
Version 1
Created by
Image of Elena Coccia, Icahn School of Medicine at Mount Sinai
Elena CocciaIcahn School of Medicine at Mount Sinai
Protocol
Name
Startle induced negative geotaxis (SING)
Version 1
Created by
Image of Natalie Kaempf, VIB KU Leuven
Natalie KaempfVIB KU Leuven
Protocol
Name
Immunofluorescent staining of dopaminergic neurons in brains of D. melanogaster
Version 1
Created by
Image of Natalie Kaempf, VIB KU Leuven
Natalie KaempfVIB KU Leuven