A buffer solution has the function of resisting changes in pH even when adding powerful acids or bases. However, in the physiological environment the buffered system also provides cofactors for enzymatic reactions, critical salts and even essential nutrients for cells and tissues. Therefore, when trying to reproduce biological conditions in vitro, we must make the appropriate choice of the buffer. After all, it will provide the appropriate medium in which reactions will occur.
 
Neilier Junior 2020. Carbonate-Bicarbonate Buffer. protocols.iohttps://dx.doi.org/10.17504/protocols.io.bfyijpue
pH, Henderson-HasselBack, Biochemistry, Molecular Biology
 protocol  ,  
May 05, 2020
Jun 03, 2020
  • May 05, 2020
    Megan Freund
  • Jun 03, 2020
    Neilier Junior
    Universidade Federal de Viçosa
 
36586
  • ph Meter (sensitive)
  • Deionized Water
  • Sodium Carbonate
  • Sodium Bicarbonate
Wear personal protective equipment: gloves, lab coat and mask.
Organize your workspace.
Make sure all solutions and equipment are available.

Carbonate-Bicarbonate Buffer

1

pH range: pH9.2 to pH10.6

(a) 0.1 M Sodium carbonate (anhydrous), 10.6 g L-1 (M.W.: 106.0 g mol-1)
(b) 0.1 M Sodium bicarbonate, 8.4 g L-1 (M.W.: 84.0 g mol-1)

Mix sodium carbonate and sodium bicarbonate solutions in the proportions indicated.

mL of Sodium carbonate4.09.516.022.027.533.038.542.5
mL of Sodium bicarbonate46.040.534.028.022.517.011.57.5
pH9.29.49.69.810.010.210.410.6

2
Adjust the final volume to 200 mL with deionized water.

3
Adjust the final pH using a sensitive pH meter.