Carbonate-Bicarbonate Buffer
Jun 03, 2020

Carbonate-Bicarbonate Buffer
In 1 collection

  • 1Universidade Federal de Viçosa
Abstract
A buffer solution has the function of resisting changes in pH even when adding powerful acids or bases. However, in the physiological environment the buffered system also provides cofactors for enzymatic reactions, critical salts and even essential nutrients for cells and tissues. Therefore, when trying to reproduce biological conditions in vitro, we must make the appropriate choice of the buffer. After all, it will provide the appropriate medium in which reactions will occur.
PROTOCOL citation
Neilier Junior 2020. Carbonate-Bicarbonate Buffer. protocols.iohttps://dx.doi.org/10.17504/protocols.io.bfyijpue
Keywords
pH, Henderson-HasselBack, Biochemistry, Molecular Biology
License
This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Created
May 05, 2020
Last Modified
Jun 03, 2020
Ownership history
  • May 05, 2020
    Megan Freund
  • Jun 03, 2020
    Neilier Junior
    Universidade Federal de Viçosa
PROTOCOL integer ID
36586
Parent protocols
Part of collection
Buffers for Use in Biological Systems
MATERIALS TEXT
  • ph Meter (sensitive)
  • Deionized Water
  • Sodium Carbonate
  • Sodium Bicarbonate
Safety warnings
Wear personal protective equipment: gloves, lab coat and mask.
Abstract
A buffer solution has the function of resisting changes in pH even when adding powerful acids or bases. However, in the physiological environment the buffered system also provides cofactors for enzymatic reactions, critical salts and even essential nutrients for cells and tissues. Therefore, when trying to reproduce biological conditions in vitro, we must make the appropriate choice of the buffer. After all, it will provide the appropriate medium in which reactions will occur.
Organize your workspace.
Make sure all solutions and equipment are available.

Carbonate-Bicarbonate Buffer

1

pH range: pH9.2 to pH10.6

(a) 0.1 M Sodium carbonate (anhydrous), 10.6 g L-1 (M.W.: 106.0 g mol-1)
(b) 0.1 M Sodium bicarbonate, 8.4 g L-1 (M.W.: 84.0 g mol-1)

Mix sodium carbonate and sodium bicarbonate solutions in the proportions indicated.

mL of Sodium carbonate4.09.516.022.027.533.038.542.5
mL of Sodium bicarbonate46.040.534.028.022.517.011.57.5
pH9.29.49.69.810.010.210.410.6

2
Adjust the final volume to 200 mL with deionized water.

3
Adjust the final pH using a sensitive pH meter.