A buffer solution has the function of resisting changes in pH even when adding powerful acids or bases. However, in the physiological environment the buffered system also provides cofactors for enzymatic reactions, critical salts and even essential nutrients for cells and tissues. Therefore, when trying to reproduce biological conditions in vitro, we must make the appropriate choice of the buffer. After all, it will provide the appropriate medium in which reactions will occur.
Neilier Junior 2020. Carbonate-Bicarbonate Buffer. protocols.iohttps://dx.doi.org/10.17504/protocols.io.bfyijpue
KeywordspH, Henderson-HasselBack, Biochemistry, Molecular Biology
This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
CreatedMay 05, 2020
Last ModifiedJun 03, 2020
- May 05, 2020Megan Freund
- Jun 03, 2020Neilier JuniorUniversidade Federal de Viçosa
PROTOCOL integer ID36586
Part of collectionBuffers for Use in Biological Systems
- ph Meter (sensitive)
- Deionized Water
- Sodium Carbonate
- Sodium Bicarbonate
Wear personal protective equipment: gloves, lab coat and mask.
Organize your workspace.
Make sure all solutions and equipment are available.
Mix sodium carbonate and sodium bicarbonate solutions in the proportions indicated.
|mL of Sodium carbonate||4.0||9.5||16.0||22.0||27.5||33.0||38.5||42.5|
|mL of Sodium bicarbonate||46.0||40.5||34.0||28.0||22.5||17.0||11.5||7.5|
Adjust the final volume to 200 mL with deionized water.
Adjust the final pH using a sensitive pH meter.