Protocol Citation: PMAT0001 2020. Calibrating a pH meter. protocols.io https://protocols.io/view/calibrating-a-ph-meter-bng6mbzeVersion created by PMAT0001
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
Created: October 15, 2020
Last Modified: October 15, 2020
Protocol Integer ID: 43262
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Abstract
Step-by-step instructions on calibrating a pH meter
Materials
MATERIALS
Water refers to sterilized deionized water
Potassium ChlorideSigma AldrichCatalog #P9541
HyperSep™ SLE Cartridge (pH 7), 20g/60mLThermo FisherCatalog #60109-20000-60-7
Orion™ pH 4.01 Buffer, Individual Use, Pink, 25 Pouches, pH 4.01 Buffer, Color Coded Pink, 25 PouchesThermo FisherCatalog #910425
pH meter
Main steps
Main steps
Press “Cal”
Press “✔️”
Dip in pH 4
Press “✔️”
Rinse pH meter
Dip in pH 7
Press “✔️”
Dip into sample; Note percentage purity as well
Important notes
Important notes
- When liquid level falls way below the "hole" at the top, replenish with some KCl
- After finishing experiments for the day, rinse pH meter, secure top with parafilm and put in KCl (parafilm to the KCl storage as well)