Mar 16, 2024
- anita.adami 1
- 1Laboratory of Molecular Neurogenetics, Department of Experimental Medical Science, Wallenberg Neuroscience Center and Lund Stem Cell Center, BMC A11, Lund University, 221 84 Lund, Sweden.
External link: https://doi.org/10.1126/sciadv.adh9543
Protocol Citation: anita.adami 2024. Bulk RNA sequencing. protocols.io https://dx.doi.org/10.17504/protocols.io.36wgqjqbkvk5/v1
Manuscript citation:
Raquel Garza, Diahann A. M. Atacho, Anita Adami, Patricia Gerdes, Meghna Vinod, PingHsun Hsieh, Ofelia Karlsson, Vivien Horvath, Pia A. Johansson, Ninoslav Pandiloski, Jon Matas-Fuentes, Annelies Quaegebeur, Antonina Kouli, Yogita Sharma, Marie E. Jönsson, Emanuela Monni, Elisabet Englund, Evan E. Eichler, Molly Gale Hammell, Roger A. Barker, Zaal Kokaia, Christopher H. Douse, Johan Jakobsson (2023) LINE-1 retrotransposons drive human neuronal transcriptome complexity and functional diversification.Science Advances doi: 10.1126/sciadv.adh9543
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: March 04, 2023
Last Modified: June 01, 2024
Protocol Integer ID: 78066
Keywords: ASAPCRN, bulk rna, rna, bulk, protocol
Funders Acknowledgements:
Aligning Science Across Parkinson's through the Michael J. Fox Foundation for Parkinson's Research
Grant ID: ASAP-000520
Swedish Research Council
Grant ID: 2018-02694
Swedish Brain Foundation
Grant ID: FO2019-0098
Cancerfonden
Grant ID: 190326
Barncancerfonden
Grant ID: PR2017-0053
NIHR Cambridge Biomedical Research Centre
Grant ID: NIHR203312
Swedish Society for Medical Research
Grant ID: S19-0100
National Institutes of Health
Grant ID: HG002385
Swedish Research Council
Grant ID: 2021-03494
Swedish Research Council
Grant ID: 2020-01660
Abstract
This protocol describes how to perform bulk RNA sequencing
Troubleshooting
Bulk RNA sequencing
Total RNA was isolated from nuclei (Nuclear Extraction from Tissue for FACS), cell culture samples, or tissue using the RNeasy Mini Kit (Qiagen).
Libraries were generated using Illumina TruSeq Stranded mRNA library prep kit (poly-A selection) and sequenced on a NextSeq500 (PE 2×150bp).