Feb 25, 2022
Bristol’s Modified Medium
This document is a draft, published without a DOI.
- 1University of Michigan - Ann Arbor
- Duffy Lab, EEB, University of Michigan
Document Citation: Kristel Sanchez 2022. Bristol’s Modified Medium. protocols.io https://protocols.io/view/bristol-s-modified-medium-b5mqq45w
License: This is an open access document distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Created: February 25, 2022
Last Modified: February 25, 2022
Document Integer ID: 58768
Abstract
This recipe is used to culture algae of the genus Ulothrix in the lab. However, in can be used to grow other organisms such as cyanobacteria, chrysophyta, euglenophyta and other green algae.
Bristol’s Modified Medium
Author: Kristel Sánchez
Summary
This recipe is used to culture algae of the genus Ulothrix in the lab. However, in can be used to grow other organisms such as cyanobacteria, chrysophyta, euglenophyta and other green algae.
Materials
| A | B | C | |
| Apparatus & Equipment | Labware | Reagents | |
| Autoclave | 500 mL polyethylene bottles | Sodium nitrate, NaNO3 | |
| Aluminum foil | Graduated cylinder | Calcium chloride, CaCl2•2H2O | |
| Autoclave tape | 500 mL beakers | Magnesium Sulfate, MgSO4•7H2O | |
| Analytical balance | Stirring magnet | Potassium phosphate dibasic, K2HPO4 | |
| Weight boats or paper | 5L glass aspirator bottle | Potassium phosphate monobasic, KH2PO4 | |
| Rubber cap for 5L | Sodium chloride, NaCl | ||
| Rubber hose | UTEX GR+/NH4 Medium soil water | ||
| spatula | Iron (III) chloride, FeCl3 |
Primary Stock Solutions
These solutions are prepared by dissolving the prescribed amount of chemical into 400mL of MilliQ water. Store these solutions in polyethylene bottles at 4°C.
| A | B | C | D | |
| Compound | Symbol | Grams (g) | Final concentration mg/L | |
| Sodium nitrate | NaNO3 | 10 | 0.025 | |
| Calcium chloride | CaCl2•2H2O | 1 | 0.0025 | |
| Magnesium sulfate | MgSO4•7H2O | 3 | 0.0075 | |
| Potassium phosphate dibasic | K2HPO4 | 3 | 0.0075 | |
| Potassium phosphate monobasic | KH2PO4 | 7 | 0.0175 | |
| Sodium chloride | NaCl | 1 | 0.0025 |
1% Iron chloride solution
Combine 1g of iron (III) chloride in 100mL of MilliQ water. Store in polyethylene bottle at 4°C.
Final solution
Obtain an aspirator bottle that has a rubber hose and clamp already attached to it and add 1000 mL of MilliQ water. Next, add 50 mL of each primary stock solution into the aspirator media culture bottle. Add five drops of 1% iron (III) chloride solution and 200mL of soil water. Fill the bottler until the 5L mark with MilliQ water. Place the rubber stopper laterally (so it does not completely plug the top opening of the aspirator bottle) and completely cover the top with aluminum foil. Cover the end of the rubber hose with aluminum foil (or connect it to the second aspirator bottle if setting up a new culture).Autoclave the mixture by selecting program number 2 in the autoclaves found in the 4 or 5thfloor of the BSB building (or at 121 °C for 15 min). Once autoclave program has run, remove the bottles carefully from autoclave using the autoclave gloves. Let the liquid and bottles to cool down before using.