Mar 26, 2020

Public workspaceBrain-wide delivery of Janelia Fluor HaloTag ligand dyes for tracking protein turnover in mice V.1

DOI
dx.doi.org/10.17504/protocols.io.bd8gi9tw
  • 1HHMI Janelia Research Campus;
  • 2Janelia Research Campus
Boaz Mohar
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DOI: dx.doi.org/10.17504/protocols.io.bd8gi9tw
Collection Citation: Boaz Mohar, Karel Svoboda 2020. Brain-wide delivery of Janelia Fluor HaloTag ligand dyes for tracking protein turnover in mice. protocols.io https://dx.doi.org/10.17504/protocols.io.bd8gi9tw
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: In development
We are still developing and optimizing this protocol
Created: March 26, 2020
Last Modified: March 26, 2020
Collection Integer ID: 34792
Abstract
These protocols are part of a yet unpublised paper by the same title.

Files
Protocol
Icon representing the file Preparation of JF dye for retro-orbital injection in mice
Name
Preparation of JF dye for retro-orbital injection in mice
Version 1
, HHMI Janelia Research Campus
Boaz MoharHHMI Janelia Research Campus
Protocol
Icon representing the file Retro-orbital injection of virus or dye in mice
Name
Retro-orbital injection of virus or dye in mice
Version 1
, HHMI Janelia Research Campus
Boaz MoharHHMI Janelia Research Campus
Protocol
Icon representing the file Protocol for mouse perfusion with dye, DAPI staining, and slide preparation
Name
ForkProtocol for mouse perfusion with dye, DAPI staining, and slide preparation
Version 1
, HHMI Janelia Research Campus
Boaz MoharHHMI Janelia Research Campus
Protocol
Icon representing the file Mesuring JF dye kinetics in the brain of C57/Bl mice
Name
Mesuring JF dye kinetics in the brain of C57/Bl mice
Version 1
, HHMI Janelia Research Campus
Boaz MoharHHMI Janelia Research Campus