May 15, 2025

Public workspaceBouabid, S. et al. (2025) "Distinct spatially organized striatum-wide acetylcholine dynamics for the learning and extinction of Pavlovian associations" V.1

Bouabid, S. et al. (2025) "Distinct spatially organized striatum-wide acetylcholine dynamics for the learning and extinction of Pavlovian associations"
  • Safa Bouabid1,2,
  • Liangzhu Zhang1,
  • Mai-Anh Vu1,2,
  • Kylie Tang1,
  • Benjamin M. Graham1,
  • Christian A. Noggle1,
  • Mark Howe1,2
  • 1Department of Psychological & Brain Sciences, Boston University, Boston, MA, USA;
  • 2Aligning Science Across Parkinson's (ASAP) Collaborative Research Network, Chevy Chase, MD, 20815, USA
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Collection CitationSafa Bouabid, Liangzhu Zhang, Mai-Anh Vu, Kylie Tang, Benjamin M. Graham, Christian A. Noggle, Mark Howe 2025. Bouabid, S. et al. (2025) "Distinct spatially organized striatum-wide acetylcholine dynamics for the learning and extinction of Pavlovian associations". protocols.io https://dx.doi.org/10.17504/protocols.io.n2bvjdx65vk5/v1
Manuscript citation:
Bouabid, S., Zhang, L., T. Vu, MA. et al. Distinct spatially organized striatum-wide acetylcholine dynamics for the learning and extinction of Pavlovian associations. Nat Commun 16, 5169 (2025). https://doi.org/10.1038/s41467-025-60462-5
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: April 07, 2025
Last Modified: May 15, 2025
Collection Integer ID: 126263
Keywords: Dopamine, Mouse, Behaviour, acetylcholine, photometry, multi-fiber array, viral vectors, Pavlovian, striatum, wide acetylcholine dynamics for the learning, extinction of pavlovian association, pavlovian association, wide acetylcholine dynamic, organized striatum
Funders Acknowledgements:
Aligning Science Across Parkinson's (ASAP)
Grant ID: ASAP-020370
Files
Protocol
Icon representing the file Fabrication and calibration of multi-fiber arrays to monitor dopamine release in the mouse striatum
Name
ForkFabrication and calibration of multi-fiber arrays to monitor dopamine release in the mouse striatum
Version 1
, University of Oxford
Cláudia C. MendesUniversity of Oxford
Protocol
Icon representing the file Stereotaxic injections of viral vectors and chronic optical fiber implants in mouse brains
Name
Stereotaxic injections of viral vectors and chronic optical fiber implants in mouse brains
Version 1
, University of Oxford
Cláudia C. MendesUniversity of Oxford
Protocol
Icon representing the file Multi-fiber photometry in head-fixed mice performing a dual-cue delay Pavlovian association
Name
Multi-fiber photometry in head-fixed mice performing a dual-cue delay Pavlovian association
Version 1
, University of Oxford
Cláudia C. MendesUniversity of Oxford
Protocol
Icon representing the file Micro-CT scanning for post-implant localization of multi-fiber arrays in mouse striatum
Name
ForkMicro-CT scanning for post-implant localization of multi-fiber arrays in mouse striatum
Version 1
, University of Oxford
Cláudia C. MendesUniversity of Oxford
Protocol
Icon representing the file Confocal microscopy to assess TelC and iGluSnFR expression in fixed mouse brain slices
Name
Confocal microscopy to assess TelC and iGluSnFR expression in fixed mouse brain slices
Version 1
, University of Oxford
Cláudia C. MendesUniversity of Oxford
Protocol references
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Vu, M.-A.T., Brown, E.H., Wen, M.J., Noggle, C.A., Zhang, Z., Monk, K.J., Bouabid, S., Mroz, L., Graham, B.M., Zhuo, Y., et al. Targeted micro-fiber arrays for measuring and manipulating localized multi-scale neural dynamics over large, deep brain volumes during behavior. Neuron 112 , 909-923.e9 (2024).

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Patriarchi, T., Cho, J.R., Merten, K., Howe, M.W., Marley, A., Xiong, W.-H., Folk, R.W., Broussard, G.J., Liang, R., Jang, M.J., et al. (2018). Ultrafast neuronal imaging of dopamine dynamics with designed genetically encoded sensors.Science 360 , eaat4422.
Zhang, Y., Zhao, S., Rodriguez, E., Takatoh, J., Han, B.-X., Zhou, X., and Wang, F. (2015). Identifying local and descending inputs for primary sensory neurons. J Clin Invest 125 , 3782–3794.
Marvin, J.S., Scholl, B., Wilson, D.E., Podgorski, K., Kazemipour, A., Müller, J.A., Schoch, S., Quiroz, F.J.U., Rebola, N., Bao, H., et al. (2018). Stability, affinity, and chromatic variants of the glutamate sensor iGluSnFR. Nat Methods 15 , 936–939.


Wang, Q., Ding, S.-L., Li, Y., Royall, J., Feng, D., Lesnar, P., Graddis, N., Naeemi, M., Facer, B., Ho, A., et al. (2020). The Allen Mouse Brain Common Coordinate Framework: A 3D Reference Atlas. Cell 181, 936-953.e20. 10.1016/j.cell.2020.04.007.