Nov 09, 2022
Bionic sensing system and characterization of exhaled nitric oxide detection based on canine olfaction
- 时野坪 1
- 1Harbin University of Science and Technology
External link: https://doi.org/10.1371/journal.pone.0279003
Protocol Citation: 时野坪 2022. Bionic sensing system and characterization of exhaled nitric oxide detection based on canine olfaction. protocols.io https://dx.doi.org/10.17504/protocols.io.j8nlkwwp1l5r/v1
Manuscript citation:
Sun P, Shi Y, Shi Y (2022) Bionic sensing system and characterization of exhaled nitric oxide detection based on canine olfaction. PLoS ONE 17(12): e0279003. doi: 10.1371/journal.pone.0279003
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: November 08, 2022
Last Modified: November 09, 2022
Protocol Integer ID: 72449
Keywords: characterization of exhaled nitric oxide detection, exhaled nitric oxide detection, bionic sensing system, bionic chamber, relative optimal bionic chamber size, canine olfaction section, sensor, response voltages of the no sensor, air pump, pumping flow capacity of the air pump
Abstract
section 1:Design the relative optimal bionic chamber size and the pumping flow capacity of the air pump. The chamber's length, inner diameter, and pump flow capacity are designed, experimented and analyzed.
section 2:The chamber's relatively optimal sieve plate structure is obtained through the design, experiment, and analysis of the number of sieve plates, the number of air holes, and the diameter of air holes in the bionic chamber.
section 3:The response voltages of the NO sensor in three bionic chambers were collected in the same experimental environment.
Troubleshooting
Bionic chamber design
The experimental factors are reasonably designed and investigated through the orthogonal test method, and the level table of orthogonal test factors is established .
Bionic chambers printed in 3D according to orthogonal design results.
Under the same conditions, nine experiments were carried out according to the orthogonal experimental design table.
The sensor's output voltage is collected using a data acquisition card.
The voltage data is processed by Matlab software by moving the average.
The stable response signal is averaged.
The same group experiment was repeated three times.
Optimal design of bionic chamber sieve plates
The experimental factors are reasonably designed and investigated through the orthogonal test method, and the level table of orthogonal test factors is established .
Bionic chambers printed in 3D according to orthogonal design results.
Under the same conditions, nine experiments were carried out according to the orthogonal experimental design table.
The sensor's output voltage is collected using a data acquisition card.
The voltage data is processed by Matlab software by moving the average.
The stable response signal is averaged
The same group experiment was repeated three times.
The response voltages of sensor in three bionic chambers
Clean the experimental environment with nitrogen before the experiment and ensure that sensors used in each experiment have the same baseline voltage.
Inject different volumes (0.35ml, 0.7ml, 1.05ml) of NO gas into the closed box. The concentration of NO gas in the closed box after mixing is 15ppb, 30ppb, and 45ppb.
The sensor was placed in bionic chambers, and the pumping flow capacitywas set to 1L/min.
The sensor's output voltage is collected using a data acquisition card.
The voltage data is processed by Matlab software by moving the average.
The stable response signal is averaged.
The same group experiment was repeated three times.