Here we present a bioinformatic pipeline for dissecting transcriptional regulation of neural differentiation process. Single cell RNA-seq and bulk ATAC-seq with two biological replicates was applied to the indicated cell stages including human induced pluripotent stem cells (hiPSCs), embryoid body (EB), early rosettes (Ros-E), late rosettes (Ros-L), neural progenitor cells (NPCs), and the original somatic fibroblasts (Fib) for a deeper understanding of the regulatory mechanisms driving the differentiation of the neural lineage. This pipeline could be applied to study transcriptome and regulome dynamics of other lineages.