We cut 1 g of each sample in such way that it contained the cuticle from one side of the sample. We homogenized it with 35 ml of the methanol 100%, and left them in ultrasonic 6 l bath during 30 min at room temperature. Methanol extracts obtained were filtered, placed in amber bottles to avoid degradation by light, and stored at \u201320\u00b0C until processed. We used Waters 717 liquid chromatograph with autosampler, Waters 2487 HPLC Absorbance UV-Vis Detector, Waters 1525 Binary HPLC Pump, Waters control module with SAT\/IN Bus (Waters, Milford, MA, USA), Symmetry HPLC C18 column (particle size 5 \u00b5m, length 250 mm, internal \u00d8 = 4.6 cm; Waters, Milford, MA, USA). We filtered the extracts with 0.45 \u00b5m pore size nylon-membrane filter. To obtain the calibration curves we used standards for salicylic acid (SAL), 4-hydroxybenzoic acid (4-HBA), chlorogenic acid (CGA), and quercetin (QUE), all supplied by Sigma-Aldrich. The mobile phase consisted of 0.1% v\/v acetic acid (A) together with 100% acetonitrile (B). For the mobile phase A, we dissolved 1ml of glacial acetic acid with HPLC water, until 1 L. For the mobile phase B we used 100% acetonitrile. We filtered both mobile phases with 0.45 \u00b5m nylon membrane. We degasified them with ultrasonic bath during 30 min. We put the column temperature at 25\u00b0C, used the 254 nm UV detector, established the flow of the mobile phase at 0.2\u20130.8 mL\/min, injection volume at 8 \u00b5L, and run time at 35 min. To wash the piston seals we used MeOH : H2O (60 : 50). To quantify the four compounds, we used the calibration curves prepared out of 10 mg stock solution in 5 mL of MeOH. We took known concentrations from these solutions to obtain the relationship between the area corresponding to absorbance and concentration of each metabolite.