Aug 27, 2020
Version 2
BG11 hypersaline medium V.2
- 1Massachusetts Institute of Technology
- Bosak Lab
Protocol Citation: Tanja Bosak 2020. BG11 hypersaline medium. protocols.io https://dx.doi.org/10.17504/protocols.io.bkcmksu6
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: August 26, 2020
Last Modified: August 27, 2020
Protocol Integer ID: 41069
Add the following to a beaker and dissolve using a stir bar on a magnetic stir plate.
| BG11 hypersaline medium | amount | unit | |
| initial Milli-Q water | 968 | ml | |
| NaCl | 49.8 | g | |
| NaNO3 | 1.5 | g | |
| Na2CO3 | 0.02 | g | |
| KCl | 1.3867 | g | |
| MgCl2*6H2O | 8.569 | g | |
| MgSO4*7H2O | 6.4998 | g | |
| CaCl2*2H2O | 2.042 | g | |
| Stock A | 10 | ml | |
| Stock B | 10 | ml | |
| Stock C | 10 | ml | |
| Stock 5 (trace metals) | 1 | ml | |
| Vitamins | 1 | ml |
Add Milli-Q water to bring total volume to 1 L.
Adjust pH to ~7 - 7.4
Filter sterilize near Bunsen burner or in sterile biohood.
STOCK SOLUTIONS
| Stock A | Concentration (g/L) | |
| Na2MG EDTA | 0.1 | |
| Ferric ammonium citrate | 0.6 | |
| Citric acid*1H2O | 0.6 | |
| CaCl2*2H2O | 3.6 | |
| Add up to 1L of dH20 |
| Stock B | Concentration (g/L) | |
| MgSO4*7H2O | 7.5 | |
| Add up to 1L of dH20 |
| Stock C | Concentration (g/L) | |
| K2HPO4*3H2O | 4 | |
| Add up to 1L of dH20 |
| Stock 5 | Concentration (g/L) | |
| H3BO3 | 2.86 | |
| MnCl2*4H2O | 1.81 | |
| ZnSO4*7H2O | 0.222 | |
| CuSO4*5H2O | 0.079 | |
| CoCl2*6H2O | 0.05 | |
| NaMoO4*2H2O | 0.391 | |
| Add up to 1L of dH20 |