Please contact Dr. Steven Wilhelm (wilhelm@utk.edu) for additional information regarding this protocol.Adapted from the original publication Rippka, R., DeRulles, J., Waterbury, J. B., Herdman, M. & Stanier, R. Y. Generic assignments, strain histories and properties of pure cultures of cyanobacteria. Journal of General Microbiology 111, 1-61 (1979).