Protocol Citation: Yin-Tse Huang, Tina 2022. Beetle rearing media. protocols.io https://dx.doi.org/10.17504/protocols.io.3byl4b62jvo5/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: In development
We are still developing and optimizing this protocol
Created: June 13, 2022
Last Modified: September 13, 2023
Protocol Integer ID: 64541
Abstract
ambrosia beetle rearing
The recipe produces enough medium to fill approximately 18 culture tubes.(12cm)
First, add powder or solid
| A | B | |
| component | amount | |
| streptomycin | 0.175 g | |
| Wesson's salt mixture | 0.625 g | |
| yeast | 4 g | |
| corn starch | 4 g | |
| casein | 5 g | |
| agar | 15 g | |
| sucrose | 2.5 g | |
| redbay sawdust | 100 g |
Second, add liquid
| A | B | |
| component | amount | |
| wheat germ oil | 1.25ml | |
| 95％ ethanol | 2.5ml | |
| deionized water | 290ml |
All ingredients were mixed thoroughly, then put cotton plugs into the mouth of the filled tubes.
The culture tubes was autoclaved at 121 °C for 30 min.
Put culture tubes to dry 2 days in oven at 55°C.
(cotton plugs don't remove)