Generally, growth assays for filamentous fungi have been performed on solid media, either as dilution series or spot tests. However, the solid media environment does not accurately mimic the environment encountered during infection (ie the mammalian lung). Previously, we have developed a methodology to perform liquid growth assays in time for A. fumigatus and other filamentous fungi including analysis via mathematical modelling.This protocol is designed for Aspergillus species (and other filamentous fungi) to generate growth curves in liquid media in a 96-well plate. This can be done in high-throughput to generate 96 growth curves per run.