This protocol presents a simple modification of the SMART-seq2 (Picelle et al, 2014) and SCRB-seq (Soumillon et al, 2014) protocols, with the goal of combining the sensitivity of SMART-Seq2 with the improved cost and throughput of 3' single cell protocols. Reverse transcription (RT) and PCR proceed as in SMART-Seq2, but using 3' barcoded oligos from SCRB-seq. While most barcoded methods (including SCRB-seq) pool cells together after RT, we perform PCR individually in each well, as this negates the need for an additional exonuclease and purification step, and therefore maintains the same molecular sensitivity as SMART-Seq2. However, we pool amplified cDNA from single wells immediately after PCR, and generate a single tagmentation-based 3' cDNA library. While this protocol therefore has advantages in both sensitivity and throughput, the absence of an exonuclease step prior to PCR disables the use of unique molecular identifiers for quantification.