A general process for preparing, collecting, and analyzing data is as follows:
Start the SoftMax Pro Software.
Select an instrument: Check instrument connection status --> it should be connected with a green check. If showing disconnected: click on the instrument icon, the instrument connection dialog is displayed. In the available instrument list wait to display Offiline Spectra Max iD3, select Spectra Max iD3, click OK
It should connect --> for troubleshooting refer to Softmax Pro User Guide.
Choose a data acquisition protocol. You can use an existing protocol or create a new one.
--> To use an existing protocol:
1. Click the Application button to open the Application menu.
2. Select Open. The Data Files file type is selected by default.
3. Select a protocol file type from the list.
SoftMax Pro Software 7 can open files created by version 4.0 or newer.
When you open a file from a previous version, it is converted to SoftMax Pro Software
4. Navigate to the protocol file you want to open.
An untitled file opens in the workspace with the settings of the selected protocol applied to
it. Before using the protocol to collect data, save it as a data file.
--> To set up a new protocol:
Define the instrument settings to use with the protocol. To open the Settings dialog:
1. Click Settings on the Home tab in the ribbon or in the toolbar at the top of the
The available options in the Settings dialog has two methods available for configuring a microplate read.
The Standard View uses a table-like design, with selectable categories where you can
configure your settings. The Acquisition View uses a workflow design, with drag-and-drop tools to configure your settings.
Select parameters for your application:
1- Read modes: ABS, FL or LUM
2- Read type: Endpoint, kinetic, well scan or spectrum
3- Categories: --> type wavelengths --> select the plate type applied to your measurement from the list --> Read area: select specific wells or entire plate --> Path check: uncheck--> Shake: check and select specific settings (time in seconds, intensity, and shake mode) or leave empty if no shaking is needed before measurement --> More settings: define read order row or columns-wise.
After defining the settings for the protocol, click OK to close the Settings dialog.
4- Click on the open door icon
5- Insert your plate, A1 position should be at the top left corner
6- Click on the Read icon in the software --> door will close automatically and measurements will start.
7- Click the Open door icon
9- Click the close door icon
--> To save and export the acquired data:
1-Click on home --> save as --> spd
2- Click on home --> export --> plate --> txt or xls--> choose folder location --> save
Close Softmax Pro software