Dec 28, 2023
Baermann Funnel Extraction
- 1The Ohio State University
- OSU Soybean Pathology and Nematology
Protocol Citation: Casey A Schlenker 2023. Baermann Funnel Extraction. protocols.io https://dx.doi.org/10.17504/protocols.io.n2bvj31rxlk5/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: December 28, 2023
Last Modified: February 28, 2024
Protocol Integer ID: 92763
Abstract
Baermann Funnel Extraction
Materials
Materials for sample preparation:
- Glass funnel
- Funnel stand
- Filter paper
- Tubing
- 2mm mesh sieve
- DI water bottles
Sample Preparation
Sample Preparation
6d
6d
Sieve soil to 2mm
Weigh out 50 g soil in a weigh boat
Place tissue paper on top of Baermann funnel apparatus
Place weighed and sieved soil on top of filter paper
Fill Baermann funnel with DI water using a DI water bottle so that soil sample is saturated but not submerged in water
Cover all Baermann funnels with a plastic Uline
At 24:00:00 and 48:00:00 mark, check soil and refill any funnels with decreased water levels to prevent the soil from drying out.
2d
At 72:00:00 empty funnel over a 25um mesh sieve to collect nematodes
1d
Transfer the nematodes that are on the sieve into a labelled centrifuge tube using a hot fixative
Protocol
NAME
Fixative preparation for Baermann funnel extractionCREATED BY
Casey A Schlenker
Collect soil from top of Baermann funnel; dry and weigh to get gravimetric soil moisture content
Count the entire sample at 50x magnification using a dissecting scope
Identify 100 nematodes