Jun 19, 2019

Public workspaceBacterial Media Preparation

DOI
dx.doi.org/10.17504/protocols.io.4aigsce
  • 1Dartmouth College, Ackerman Lab
  • Ackerman Lab
Andrew Crowley
Icon indicating open access to content
QR code linking to this content
DOI: dx.doi.org/10.17504/protocols.io.4aigsce
Protocol Citation: Andrew Crowley 2019. Bacterial Media Preparation. protocols.io https://dx.doi.org/10.17504/protocols.io.4aigsce
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: June 17, 2019
Last Modified: June 19, 2019
Protocol Integer ID: 24618
Keywords: media, LB, TSB, bacterial culture
Abstract
Protocol for the preparation of bacterial culture and recovery media, with options for several types of antibiotics.

Default scale - 1 L
Materials
STEP MATERIALS
ReagentKanamycinResearch Products International Corp (RPI)Catalog #K22000-25.0
ReagentAmpicillinResearch Products International Corp (RPI)Catalog #A40040-25.0
ReagentLBResearch Products International Corp (RPI)Catalog #L24400-2000.0
ReagentTryptic Soy BrothFisher ScientificCatalog #DF0370-07-5
Protocol materials
ReagentKanamycinResearch Products International Corp (RPI)Catalog #K22000-25.0
ReagentAmpicillinResearch Products International Corp (RPI)Catalog #A40040-25.0
ReagentLBResearch Products International Corp (RPI)Catalog #L24400-2000.0
ReagentTryptic Soy BrothFisher ScientificCatalog #DF0370-07-5
ReagentLBResearch Products International Corp (RPI)Catalog #L24400-2000.0
ReagentKanamycinResearch Products International Corp (RPI)Catalog #K22000-25.0
ReagentAmpicillinResearch Products International Corp (RPI)Catalog #A40040-25.0
ReagentTryptic Soy BrothFisher ScientificCatalog #DF0370-07-5
Select the type of media from the list below:
Step case

Luria broth (LB)
5 steps

Add powdered LB (Amount25 g ) to deionized water (Amount1 L )


ReagentLBResearch Products International Corp (RPI)Catalog #L24400-2000.0

Note
n.b.

Particulates may remain after addition, but these will dissolve during the autoclave cycle



Autoclave using liquid cycle Duration00:15:00

Allow media to cool to Temperature50 °C

Select antibiotic from the list below:
Step case

Kanamycin
1 step

Add Amount50 mg kanamycin and mix thoroughly


ReagentKanamycinResearch Products International Corp (RPI)Catalog #K22000-25.0