Feb 27, 2023

Public workspaceBacterial isolation and genomic DNA extraction of Mycobacterium avium from pig lymph nodes

DOI
https://dx.doi.org/10.17504/protocols.io.bzbtp2nn
Bacterial isolation and genomic DNA extraction of Mycobacterium avium from pig lymph nodes
  • Tetsuya Komatsu1,
  • Kenji Ohya2,
  • Justice Opare Odoi3,
  • Shota Suganuma2,
  • Kotaro Sawai2,
  • Takayuki Wada4,
  • Tomotada Iwamoto5,
  • Fumito Maruyama6
  • 1Aichi Prefectural Chuo Livestock Hygiene Service Center, Okazaki, Aichi, Japan;
  • 2Faculty of Applied Biological Sciences, Gifu University, Gifu, Gifu, Japan;
  • 3United Graduate School of Veterinary Sciences, Gifu University, Gifu, Gifu, Japan;
  • 4Graduate School of Human Life Science, Osaka City University, Osaka, Osaka, Japan;
  • 5Department of Infectious Diseases, Kobe Institute of Health, Kobe, Hyogo, Japan;
  • 6Office of Academic Research and Industry-Government Collaboration, Hiroshima University, Higashi-Hiroshima, Hiroshima, Japan
Tetsuya Komatsu
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DOI: https://dx.doi.org/10.17504/protocols.io.bzbtp2nn
External link: https://doi.org/https://doi.org/10.1101/2021.06.15.447579
Collection Citation: Tetsuya Komatsu, Kenji Ohya, Justice Opare Odoi, Shota Suganuma, Kotaro Sawai, Takayuki Wada, Tomotada Iwamoto, Fumito Maruyama 2023. Bacterial isolation and genomic DNA extraction of Mycobacterium avium from pig lymph nodes. protocols.io https://dx.doi.org/10.17504/protocols.io.bzbtp2nn
Manuscript citation:
Komatsu T, Ohya K, Ota A, Nishiuchi Y, Yano H, Matsuo K, Odoi JO, Suganuma S, Sawai K, Hasebe A, Asai T, Yanai T, Fukushi H, Wada T, Yoshida S, Ito T, Arikawa K, Kawai M, Ato M, Baughn AD, Iwamoto T, Maruyama F, Genomic features of subsp. isolated from pigs in Japan. GigaByte doi: https://doi.org/10.1101/2021.06.15.447579
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
Created: October 21, 2021
Last Modified: February 27, 2023
Collection Integer ID: 54355
Keywords: Mycobacterium avium, bacterial isolation, Genomic DNA extraction, genomic dna extraction of mycobacterium avium, pig lymph nodes mycobacterium avium subsp, mycobacterium avium, genomic dna extraction, mah from pig lymph node, dna extraction, pig lymph node, human isolate, genome, dna, potential source of infection, infection, pig
Abstract
Mycobacterium avium subsp. hominissuis (MAH) is one of the most important agents causing non-tuberculosis mycobacterial infection in human and pigs. Genome analysis on MAH of human isolates has been proceeding, however, those of pigs are limited despite its potential source of infection to human. Here, we isolated MAH from pig lymph nodes or livers and obtained their genomes.
Files
Protocol
Name
Bacterial Isolation of Mycobacterium avium
Version 1
, Aichi Prefectural Chuo Livestock Hygiene Service Center, Okazaki, Aichi, Japan
Tetsuya KomatsuAichi Prefectural Chuo Livestock Hygiene Service Center, Okazaki, Aichi, Japan
Protocol
Name
Genomic DNA extraction from Mycobacterium avium
Version 1
, Aichi Prefectural Chuo Livestock Hygiene Service Center, Okazaki, Aichi, Japan
Tetsuya KomatsuAichi Prefectural Chuo Livestock Hygiene Service Center, Okazaki, Aichi, Japan