Oct 16, 2020
Bacterial Glycerol Stocks for Long-term Storage
- 1South China University of Technology
Protocol Citation: Jiaxin Li 2020. Bacterial Glycerol Stocks for Long-term Storage. protocols.io https://dx.doi.org/10.17504/protocols.io.bm8xk9xn
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: October 10, 2020
Last Modified: October 16, 2020
Protocol Integer ID: 42999
Add 750 μL of the overnight culture to 250 μL of 60% glycerol in a 2 mL cryotube and gently mix.
Freeze the glycerol stock tube at -80°C.