Sep 29, 2025

Public workspaceATF5-inducible overexpressing human iPSCs

DOI
https://dx.doi.org/10.17504/protocols.io.4r3l21e8xg1y/v1
  • Maria Jose Perez J.1
  • 1Institut Imagine
  • Team Deleidi
Federico Bertoli
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DOI: https://dx.doi.org/10.17504/protocols.io.4r3l21e8xg1y/v1
Protocol Citation: Maria Jose Perez J. 2025. ATF5-inducible overexpressing human iPSCs. protocols.io https://dx.doi.org/10.17504/protocols.io.4r3l21e8xg1y/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: September 25, 2025
Last Modified: September 29, 2025
Protocol Integer ID: 228167
Keywords: inducible overexpressing human ipscs atf5, human ipscs atf5, inducible overexpressing
Funders Acknowledgements:
ASAP
Abstract
ATF5-inducible overexpressing human iPSCs
Troubleshooting
Transduce control human iPSCs with lentiviral vectors encoding doxycycline-inducible constructs for ATF5 overexpression (VectorBuilder ID: VB241111-1111fuf) or with an empty vector control (VectorBuilder ID: VB201122-1083bzq), both containing a neomycin resistance cassette.
After transduction, perform clonal selection with G418 at 150 μg/mL for two weeks.
Expand stable clones following selection with G418.
Use expanded stable clones for experiments without further antibiotic selection.