Intended use:Kinetic system for Aspartate Aminotransferase (AST) or Glutamic oxalacetic trasminase (GOT) determination.Teste Principle:AST catalyzes specifically the transfer of amino groups of asparctic acid to a Ketoglutarate yielding oxalacetate and Glutamate. The oxalacetate is reduced to malate by the enzyme malate dehydrogenase (MDH) and coenzyme NADH is oxidized NAD.The reduction of the absorbance at 340 nm, as consequence of NADH oxidation, is determined photometrically and is direct proportional to the AST activity in the sample.Aminotransferase determination involves the following reactions:L- Aspartate + Ketoglutarate ---(AST) → Oxalacetate + L-GlutamateOxalacetate + NADH ---(MDH)→ Malate + NAD