Apr 24, 2026
Version 4
Artificial Cerebrospinal Fluid IV (ACSF.IV) V.4
- Allen Institute for Brain Science1
- 1Allen Institute
- Allen Institute for Brain ScienceTech. support
Protocol Citation: Allen Institute for Brain Science 2026. Artificial Cerebrospinal Fluid IV (ACSF.IV). protocols.io https://dx.doi.org/10.17504/protocols.io.n92ldy5kol5b/v4Version created by Allen Institute
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: April 24, 2026
Last Modified: April 24, 2026
Protocol Integer ID: 315713
Keywords: Artificial Cerebrospinal Fluid, ACSF, RP0222, incubation, electrophysiological, recording, artificial cerebrospinal fluid iv, making artificial cerebrospinal fluid iv, incubation of fresh mouse brain slice, fresh mouse brain slice, mouse brain, electrophysiological recording, mouse, national institutes of health, brain slice, including incubation, national institute of mental health,
Abstract
This protocol describes making Artificial Cerebrospinal Fluid IV (ACSF.IV). ACSF.IV is used for multiple applications including incubation of fresh mouse brain slices prior to electrophysiological recording.
Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
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