Apr 13, 2020
Artificial Cerebrospinal Fluid I (ACSF.I)
- Allen Institute for Brain Science1
- 1Allen Institute
- BICCN / BICAN
- Allen Institute for Brain Science
Protocol Citation: Allen Institute for Brain Science 2020. Artificial Cerebrospinal Fluid I (ACSF.I). protocols.io https://dx.doi.org/10.17504/protocols.io.bezkjf4w
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: April 13, 2020
Last Modified: March 17, 2021
Protocol Integer ID: 35596
Keywords: RP0218, perfusion, transcardial, mouse, bathing, solution, brain, slicing,
Abstract
This protocol is used to prepare Artificial Cerebrospinal Fluid I (ACSF.I). ACSF.I is used for applications including transcardial perfusion prior to fresh mouse brain slicing and as a bathing solution during fresh mouse brain slicing.
Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
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