The Coy vinyl anaerobic chambre is a special device that is consructed by a purpose to create and maintain anaerobic environment. It is\u00a0made from\u00a0flexible PVC vinyl supported by tubular aluminum frame. The chambre is a totally sealed unit except two entry ports: the equipment entry port and the airlock. Airlock is used to pass items between the chambre and the outside environment without disrupting the chamber's anaerobic athmosphere. For this purpose an automatic program (P1) consisting of three evacuation and three filling steps (two with N2 and last one with gas mix) was established. Remember: A) Light objects like towels will be flying or slightly moving in an Airlock during the procedure because of an over- and down-pressure changes. Be carefull about a staff you are putting in, protect it from a damage.B) Everything that is tightly closed (bottles with media or chemicals, tubes etc.) has to be made anoxic before putting inside. Also consumables that are pre-packed (sterile serological pipettes, tips, Petri dishes) will still keep some oxygen after the Air-lock procedure. In some cases it is useful to make a small holes into packaging before to improve speed of gases exchange but it will loss the sterility.Lab personnel access the chambre to perform their operations through glove ports. It is in favour of hygiene to wear one extra single-use gloves (nitrile, cotton or any others) under.Anaerobic athmosphere inside the ten is created usually by nitrogen as innert background gas (aka purge gas) and by the hydrogen gas mix depending on the experimental demands (usually mix of N2, H2 and\u00a0if\u00a0needed CO2). However, the hydrogen\u00a0concentration inside the tent must be always kept under 5% unless the gas mixture may be explosive.\u00a0 4% of hydrogen is an optimal concentration that is non-flamable and still provides enough hydrogen for oxygen removal. In our anaerobic chambre gas mixture N2\/H2 (95%\/5% respectively) is used.