Apr 28, 2018

Public workspaceAmpicillin solution 100 mg/mL

DOI
dx.doi.org/10.17504/protocols.io.puadnse
  • 1Centro de Biotecnologia da UFRGS
Diego Bonatto
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DOI: dx.doi.org/10.17504/protocols.io.puadnse
Protocol Citation: Diego Bonatto 2018. Ampicillin solution 100 mg/mL. protocols.io https://dx.doi.org/10.17504/protocols.io.puadnse
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: April 28, 2018
Last Modified: April 28, 2018
Protocol Integer ID: 11874
Keywords: Escherichia coli, Ampicillin, Culture medium
Abstract
Ampicillin solution for E. coli strains transformed with plasmid containing AmpR gene
Materials
MATERIALS
ReagentEthanol 70% [Note: freshly prepared]
ReagentAmpicillin, sodium salt USPBio Basic Inc.Catalog #AB0028.SIZE.25g
Preparation
Preparation
Dissolve 1 g of ampicillin in 10 mL of EtOH 70% (v/v). Store solution @ -20 °C.