Intended use:Kinetic system for Alanine Aminotransferase (ALT/ GPT) activity determination.Teste Principle:ALT catalyzes the transfer of amino groups from Alanine to Ketoglutarate, yielding pyruvate and Glutamate. The pyruvate is reduced to lactate by for action of the lactate dehydrogenase (LDH) which oxidizes NADH to NAD+.The reduction of the absorbance at 340 nm, as consequence of NADH oxidation, must be photometricaly monitored, and is proportional to the ALT activity in the sample.Aminotransferase determination involves the following reactions:L-alanine + Ketoglutarate ---(ALT) → Pyruvate + L-GlutamatePyruvate + NADH ---(LDH)→ NAD + L-lactate