Sep 09, 2020
Alpaca Nanobodies Rapid Ag test
- 1Berking Biotechnology
- XPRIZE Rapid Covid Testing
Protocol Citation: Anne Berking 2020. Alpaca Nanobodies Rapid Ag test. protocols.io https://dx.doi.org/10.17504/protocols.io.bk3xkypn
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: September 08, 2020
Last Modified: September 09, 2020
Protocol Integer ID: 41815
Keywords: Nanobodies, Ag test, Procedures Rapid test, Rapid CoV-2 Test,
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Abstract
The "Rapid Ag test based on Alpaca Nanobodies" is a test developed with single domain antibodies. This type of antibody is capable of effectively recognize the Spike protein on the surface of SARS-CoV2 leading a signal that is 10 times stronger than conventional antibodies.
It takes only 8 minutes from sample to result. The procedures for using this test were designed to ensure an easy use and interpretation, making it a quick and simple test.
Materials
Equipment List
| Equipment | Supplier | Catalog # | |
| NucleoVac 24 Vacuum Manifold | Macherey-Nagel | 740299 | |
| Manifold for 24 samples | KNF | 046209/049540 | |
| Micropipette | Gilson | FA10004P |
| Kit Content | |
| Sample collection tube | |
| Solution 1. "Binding Solution" | |
| Detection Column | |
| Solution 2. "Washing Buffer" | |
| Solution 3. "Detection Reagent" | |
| Reference paper color |
Safety warnings
Processing of any sample type which could potentially be positive for SARS-CoV-2 should be conducted in BSL2+ settings. Before starting work with these samples, please contact your local EHS (environment, health and safety) or biosafety office for proper guidance on how to work with these samples
Before start
Please follow this protocol in order to use "The Alpaca Nanobodies Rapid Ag test" correctly and get the accurate results
Sample Collection
Sample Collection
5s
5s
Collect saliva in the sample collection tube.
5s
Sample Analyse
Sample Analyse
6m 5s
6m 5s
Mix saliva sample with 10mL of Solution 1 “Binding Solution” and mix gently for 2 minutes.
2m
Add Solution 1 containing the saliva to the "Detection column"
5s
Connect the column to the vacuum manifold and let the full content pass through the "Detection columns"
2m
Add 20 mL of Solution 2 "Washing Buffer".
2m
Analysis of the Results
Analysis of the Results
2m
2m
Add 100 µL of Solution 3 "Detection Reagent" and analyze after 2 minutes: blue color indicates antigen detection. Paper colour controls will be provided.
2m