Oct 24, 2020
Agarose Gel Electrophoresis
- 1Northeast Forest University
- 2020 iGEM NEFU China
Protocol Citation: Zhujun Wei 2020. Agarose Gel Electrophoresis. protocols.io https://dx.doi.org/10.17504/protocols.io.bnwsmfee
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: October 24, 2020
Last Modified: October 24, 2020
Protocol Integer ID: 43698
Abstract
The concentration of the agarose gel is 1%.
Materials
Agarose
50X TAE
Gelred
Safety warnings
Please wear gloves for the experiment, Be careful when heating, protect your hands.
Weigh 0.20 g of agarose.
Add 20 ml of TAE 1X.
Need to prepare 1X TAE with 50X TAE.
Heat up until the solution is homogeneous.
Add 1 μl Gelred into solution and mix well.
Put the solution into bed for polymerize, make sure "comb" is well placed and the solution is balanced.
20 min later, use the gel.