Jun 19, 2019
Version 2
Agar Plate Preparation V.2
- Andrew Crowley1
- 1Dartmouth College, Ackerman Lab
- Ackerman Lab
Protocol Citation: Andrew Crowley 2019. Agar Plate Preparation. protocols.io https://dx.doi.org/10.17504/protocols.io.4c3gsyn
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: June 19, 2019
Last Modified: June 19, 2019
Protocol Integer ID: 24699
Keywords: bacterial culture, LB, agar, agar plate preparation default scale, sleeves of plate, plate, default scale, scale, sleeve
Abstract
Default scale - 1 L (makes approx. 2.5 sleeves of plates)
Materials
STEP MATERIALS
AgarAlfa AesarCatalog #A10752-0E
LBResearch Products International Corp (RPI)Catalog #L24400-2000.0
KanamycinResearch Products International Corp (RPI)Catalog #K22000-25.0
AmpicillinResearch Products International Corp (RPI)Catalog #A40040-25.0
Tryptic Soy BrothFisher ScientificCatalog #DF0370-07-5
Protocol materials
AgarAlfa AesarCatalog #A10752-0E
LBResearch Products International Corp (RPI)Catalog #L24400-2000.0
KanamycinResearch Products International Corp (RPI)Catalog #K22000-25.0
AmpicillinResearch Products International Corp (RPI)Catalog #A40040-25.0
Tryptic Soy BrothFisher ScientificCatalog #DF0370-07-5
Troubleshooting
Add powdered LB (25 g ) and agar (15 g ) to deionized water (1 L )
AgarAlfa AesarCatalog #A10752-0E
LBResearch Products International Corp (RPI)Catalog #L24400-2000.0
Note
n.b.
Particulates may remain after addition, but these will dissolve during the autoclave cycle
Autoclave using liquid cycle 00:15:00
Allow media to cool to 50 °C
Select antibiotic from the list below:
Kanamycin2 steps
Add 50 mg kanamycin and mix thoroughly
KanamycinResearch Products International Corp (RPI)Catalog #K22000-25.0
Pour into sterile plates and allow to cool