Bacillus Licheniformis Enzyme Mix (1 mL per 23 mg tissue):
100 μL b. lich 100 mg/mL (10 mg/mL final) (Sigma, P5380)
1 μL 0.5 M EDTA (Sigma, A8806)
899 μL DPBS (no Ca, Mg) ThermoFisher (cat. #14190)
The enzyme is made up in DPBS (#14190). It is aliquoted and stored at -80 °C at 100 mg/mL in 100 μL aliquots..
Enzymes and BSA are made up in DPBS (no Ca, no Mg) from Thermo Fisher (14190).
Bovine Serum Albumin - Sigma (A8806).
2 mL dounce homogenizer – Bellco (1984-10002)
Centrifuge for 1.5 mL, 15 mL conicals
30 µM filters - Miltenyi (130-098-458)
Hemocytometers - InCyto Neubauer Improved (DHC-NO1-5)