License: This is an open access collection distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: February 07, 2024
Last Modified: June 01, 2024
Collection Integer ID: 94762
Keywords: ASAPCRN, proteasome, core particle, 20S proteasome, chaperone, molecular machine, multiprotein complex, POMP, PAC1, PAC2, PAC3, PAC4, propeptide, protease, structural determination by transmission electron cryo, transmission electron cryo, methods for cloning, electron cryo, cloning, microscopy, assembly intermediate, purification, structural determination
Funders Acknowledgements:
Aligning Science Across Parkinson's (ASAP)
Grant ID: ASAP‐000282
Abstract
These protocols details methods for cloning, expression, purification and structural determination by transmission electron cryo-microscopy of 20S CPs and assembly intermediates.
Guidelines
Please familiarise yourself with the laboratory safety rules and guidelines and follow these while performing the experiment. Please wear appropriate PE while performing the experiment.
Safety warnings
Please refer to the Safety Data Sheets (SDS) for health and environmental hazards. Liquid nitrogen (LN2) and other cryogens can cause severe damage to the skin and eyes. Always wear personal protective equipment when handling these cryogens.
