ADI IsletCore Protocols for the Isolation, Assessment and Cryopreservation of Human Pancreatic Islets of Langerhans for Research Purposes
Version 1
Feb 12, 2019
Open access
External link: www.isletcore.ca
Collection CitationJames Lyon, Aliya F Spigelman, Patrick E Macdonald, Jocelyn E Manning Fox 2019. ADI IsletCore Protocols for the Isolation, Assessment and Cryopreservation of Human Pancreatic Islets of Langerhans for Research Purposes. protocols.io https://dx.doi.org/10.17504/protocols.io.x3mfqk6
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: Feb 12, 2019
Last Modified: Feb 12, 2019
COLLECTION integer ID: 20301
Keywords: Human Islets, Islets of Langerhans, Cryopreservation, IsletCore

Public workspaceADI IsletCore Protocols for the Isolation, Assessment and Cryopreservation of Human Pancreatic Islets of Langerhans for Research Purposes V.1

  • 1University of Alberta
Abstract
This collection of protocols describe the processes used by the Alberta Diabetes Institute IsletCore for the isolation of human pancreatic islets of Langerhans for research. In addition, protocols related to quality control and the cryopreservation of human islets are included.

The ADI IsletCore program is described in full here:
Lyon J, Manning Fox JE, Spigelman AF, Kim R, Smith N, O'Gorman D, Kin T, Shapiro AMJ, Rajotte RV, MacDonald PE (2016) Research-focused isolation of human islets from donors with and without diabetes at the Alberta Diabetes Institute IsletCore. Endocrinology, 157(2): 560-569.
Files
Protocol
Isolation of Human Pancreatic Islets of Langerhans for Research
Name
Isolation of Human Pancreatic Islets of Langerhans for Research
Version 2
Jocelyn E Manning Fox
Jocelyn E Manning FoxUniversity of Alberta
Protocol
Human Islet Isolation Media Preparation
Name
Human Islet Isolation Media Preparation
Version 1
Jocelyn E Manning Fox
Jocelyn E Manning FoxUniversity of Alberta
Protocol
IsletCore Equipment Setup for Human Islet Isolation
Name
IsletCore Equipment Setup for Human Islet Isolation
Version 1
Jocelyn E Manning Fox
Jocelyn E Manning FoxUniversity of Alberta
Protocol
Human Pancreas Biopsy Collection, ex vivo
Name
Human Pancreas Biopsy Collection, ex vivo
Version 1
Jocelyn E Manning Fox
Jocelyn E Manning FoxUniversity of Alberta
Protocol
Human Islet Purification - COBE setup
Name
Human Islet Purification - COBE setup
Version 1
Jocelyn E Manning Fox
Jocelyn E Manning FoxUniversity of Alberta
Protocol
Human Islet Quantification and Purity Assessment
Name
Human Islet Quantification and Purity Assessment
Version 1
Jocelyn E Manning Fox
Jocelyn E Manning FoxUniversity of Alberta
Protocol
Sampling of Human Islets for Quality Control Purposes
Name
Sampling of Human Islets for Quality Control Purposes
Version 1
Jocelyn E Manning Fox
Jocelyn E Manning FoxUniversity of Alberta
Protocol
Human Islet Cryopreservation
Name
Human Islet Cryopreservation
Version 1
Jocelyn E Manning Fox
Jocelyn E Manning FoxUniversity of Alberta
Protocol
Thawing Cryopreserved Human Islets
Name
Thawing Cryopreserved Human Islets
Version 1
Jocelyn E Manning Fox
Jocelyn E Manning FoxUniversity of Alberta
Protocol
Static Glucose-stimulated Insulin Secretion (GSIS) Protocol - Human Islets
Name
Static Glucose-stimulated Insulin Secretion (GSIS) Protocol - Human Islets
Version 3
Aliya F Spigelman
Aliya F SpigelmanUniversity of Alberta