Jun 10, 2023
Adhesive Removal Test to assess sensorimotor deficits in parkinsonian mice
- natalia.lopezgonzalezdelrey 1,
- Zachary Gaertner1,
- Elizabeth Phelan1
- 1Northwestern University, Feinberg School of Medicine, Neurology Department
Protocol Citation: natalia.lopezgonzalezdelrey , Zachary Gaertner, Elizabeth Phelan 2023. Adhesive Removal Test to assess sensorimotor deficits in parkinsonian mice. protocols.io https://dx.doi.org/10.17504/protocols.io.6qpvr3x9bvmk/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: June 09, 2023
Last Modified: May 31, 2024
Protocol Integer ID: 83158
Keywords: ASAPCRN, sensorimotor deficits in parkinsonian mice, parkinsonian mice, adhesive removal test, mouse, sensorimotor deficit, mice, parkinsonian model, fine motor movement, mouth sensitivity
Funders Acknowledgements:
Aligning Science Across Parkinson's [ASAP-020600] through the Michael J. Fox Foundation for Parkinson's Research (MJFF)
Grant ID: ASAP-020600
Abstract
This behavior is used to asses fine motor movements in a mouse parkinsonian model. It checks for correct paw and mouth sensitivity (time-to-contact) and correct dexterity (time-to-remove).
Materials
MATERIALS
Transparent box (15 cm 25 cm) to be used as the testing box.
Small adhesive tape strips (0.3 cm 0.4 cm; Fig. 1).
One classic timer and one four-channel timer.
Adhesive Removal Test
Total duration: 4 days
Protocol
1h
Place the animal cage in the testing room to allow the animals to acclimate. 01:00:00
1h
Acclimating mice to testing box. 00:01:00
1m
Apply the two adhesive tape strips with equal pressure on each animal paw so that they cover the hairless part of the forepaws. Place the adhesive in alternate order (right or left) each day and randomize it within each group. 00:03:00
3m
Video record the animal’s behavior in the testing cage. 00:02:00
2m
The contact time is defined as the point that the mouse reacts to the presence of the adhesive tape strips. For each adhesive, the mouse may start by either shaking its paws and/or directly bring its paws to its mouth. Both behaviours indicate that the mouse has felt the adhesive tape and represent the end of the time to contact. Time 1: when the mouse has felt the first piece of tape. Time 2: when the second piece of tape is felt.
Continue to observe the animal’s behavior in the testing cage. The mouse may use its mouth, and to a lesser extent the other forelimb, to attempt to remove the adhesives. Time 3: when the first piece of tape is removed. Time 4: when the second piece of tape is removed. Distinguish between right and left paw for comparisons.
Return the mouse to its cage. After 24 h, repeat the procedure.Overnight
Analysis
1h
Different analysis can be made:
- Mean Time to Notice (T1,T2)
- Mean Time to Remove (T3,T4 - T1,T2)
Compare Mean Time to Notice vs Mean Time to Remove
Tip: in the post-analysis: you can distinguish between right and left paw for comparisons. This helps to detect limb differences.
Example:
- Mean Time to Notice Right (T1)
- Mean Time to remove Right (T3)
- Mean Time to Notice Left (T2)
- Mean Time to remove Left (T4)
Protocol references
Modified from: Bouet et al., 2009, doi:10.1038/nprot.2009.125