Nov 17, 2025

Public workspaceAdhesive Removal Test in Hemiparkinsonian Mice

DOI
https://dx.doi.org/10.17504/protocols.io.bp2l6z7k1gqe/v1
  • Roberto Garcia Swinburn1,
  • Ernest Arenas1
  • 1Karolinska Institute Stockholm
  • SOX6 mDA differentiation
Roberto Garcia Swinburn
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DOI: https://dx.doi.org/10.17504/protocols.io.bp2l6z7k1gqe/v1
Protocol Citation: Roberto Garcia Swinburn, Ernest Arenas 2025. Adhesive Removal Test in Hemiparkinsonian Mice. protocols.io https://dx.doi.org/10.17504/protocols.io.bp2l6z7k1gqe/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: September 11, 2025
Last Modified: November 17, 2025
Protocol Integer ID: 226948
Keywords: adhesive, parkinson, mouse, hemiparkinsonian, adhesive removal test in hemiparkinsonian mice, eye coordination in hemiparkinsonian rodent animal, hemiparkinsonian rodent animal, hemiparkinsonian mice, mice this protocol, eye coordination, complex forepaw motor coordination, mice, adhesive removal test, paw
Abstract
This protocol is able to assess complex forepaw motor coordination and paw-eye coordination in hemiparkinsonian rodent animals.
Materials
  • Green adhesive paper tape (cut into 2 × 3 mm pieces)
  • Timer or stopwatch
  • Clean testing area (quiet, well-lit)
  • Forceps (for tape placement)
  • Data recording sheet
Troubleshooting
Animal Training (Pre-Test Preparation)
Train animals for a minimum of 2 consecutive days before the testing day.
  • Perform 3 mock trials (see below for trial procedure) per paw per day (left and right paws separately).
  • Use the same tape and procedure as in the actual test.
  • Ensure animals are accustomed to both the tape and the testing procedure.
Trial Procedure
Cut adhesive paper tape into 2 mm × 3 mm pieces.
Place one piece of tape on each paw, one paw at a time, using forceps.
Start the timer when the animal attempts to remove the tape. If the animal takes longer than 60 seconds, stop the trial and resume the trial at least 15 minutes later.
Stop the timer once the tape is fully removed from the paw.
Perform 3 valid trials per paw.
Data Analysis
Calculate the average removal time across 3 trials for each paw.
Calculate Net Contralateral Time: Net Time = Ipsilateral Time − Contralateral Time
This normalization helps eliminate external influences and better visualize lateralized sensorimotor deficits.