Apr 30, 2020

Public workspaceAb Lysis Buffer

DOI
dx.doi.org/10.17504/protocols.io.bft6jnre
  • 1Oregon Health and Science University
Colin Daniel
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DOI: dx.doi.org/10.17504/protocols.io.bft6jnre
Protocol Citation: Colin Daniel 2020. Ab Lysis Buffer. protocols.io https://dx.doi.org/10.17504/protocols.io.bft6jnre
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: April 30, 2020
Last Modified: April 30, 2020
Protocol Integer ID: 36446
Materials
MATERIALS
Reagent100g Deoxycholate (Deoxycholic acid sodium salt)G-BiosciencesCatalog #DG090
ReagentNaClMerck MilliporeSigma (Sigma-Aldrich)Catalog #53014
ReagentEthylenediaminetetraacetic acidMerck MilliporeSigma (Sigma-Aldrich)Catalog #E9884
ReagentTriton X-100Fisher ScientificCatalog #BP151-100
ReagentTris BaseFisher ScientificCatalog #604204
ReagentSodium dodecyl sulfate solutionMerck MilliporeSigma (Sigma-Aldrich)Catalog #71736-100ML
Concentration20 millimolar (mM) TrisPh7.5
Concentration50 millimolar (mM) NaCl
Concentration0.5 % (v/v) Triton-X100
Concentration0.5 % (v/v) DOC
Concentration0.5 % (v/v) SDS
Concentration1 millimolar (mM) EDTA

On ice add protease and phosphotase inhibitors (Roche)