Jul 12, 2023

A tri-specific killer engager (TriKE) against mesothelin targets NK cells towards lung cancer V.2

  • 1University of Minnesota
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Collection CitationPhilippa Kennedy 2023. A tri-specific killer engager (TriKE) against mesothelin targets NK cells towards lung cancer. protocols.io https://dx.doi.org/10.17504/protocols.io.5qpvorx29v4o/v2Version created by Philippa R Kennedy
License: This is an open access  collection  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: November 03, 2022
Last Modified: July 12, 2023
Collection  Integer ID: 72224
Keywords: cancer, lung, immunotherapy, natural killer, NK cell, immunology, mesothelin targets nk cells towards lung cancer, mesothelin targets nk cell, lung cancer, trike,
Abstract
A collection of protocols associated with the publication 'A tri-specific killer engager (TriKE) against mesothelin targets NK cells towards lung cancer' by Kennedy et al.
Files
Protocol
Name
Isolation of natural killer (NK) cells from human blood products
Version 1
Created by
Image of Philippa R Kennedy, University of Minnesota
Philippa R KennedyUniversity of Minnesota
Protocol
Name
Cell line information
Version 3
Created by
Image of Philippa R Kennedy, University of Minnesota
Philippa R KennedyUniversity of Minnesota
Protocol
Icon representing the file Time of flight mass cytometry (CyTOF)
Name
Time of flight mass cytometry (CyTOF)
Version 1
Created by
Image of Philippa R Kennedy, University of Minnesota
Philippa R KennedyUniversity of Minnesota
Protocol
Name
Degranulation and cytokine production (functional assay)
Version 1
Created by
Image of Philippa R Kennedy, University of Minnesota
Philippa R KennedyUniversity of Minnesota
Protocol
Icon representing the file Proliferation assay
Name
Proliferation assay
Version 1
Created by
Image of Philippa R Kennedy, University of Minnesota
Philippa R KennedyUniversity of Minnesota
Protocol
Name
Assessing IL-15 bioavailability ("the bioassay")
Version 1
Created by
Image of Philippa R Kennedy, University of Minnesota
Philippa R KennedyUniversity of Minnesota
Protocol
Icon representing the file Time-lapse killing assay (monolayer - IncuCyte)
Name
Time-lapse killing assay (monolayer - IncuCyte)
Version 1
Created by
Image of Philippa R Kennedy, University of Minnesota
Philippa R KennedyUniversity of Minnesota