Aug 17, 2020
A simple method for purification of immunoglobulin-Y (IgY).
- 1University of the West Indies St. Augustine
- University of the West Indies
- angel.vaillant@sta.uwi.edu
Protocol Citation: Angel A Justiz-Vaillant 2020. A simple method for purification of immunoglobulin-Y (IgY).. protocols.io https://dx.doi.org/10.17504/protocols.io.bjuwknxe
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: August 17, 2020
Last Modified: August 17, 2020
Protocol Integer ID: 40566
Separate egg yolk from the egg white.
Add ten parts of distilled water to one part egg yolk.
Mix and stir slowly for 6.30 h at 4°C.
Centrifuge at 10 000 × g, at 4°C for 27 min to precipitate the lipids.
Collect the supernatant containing the IgY.
Slowly add potassium sulfate to the preparation or specimen, stirring constantly, to a final concentration of 500 mM.
Adjust pH to 7.6.
Pass the sample through a 0.45 µm filter just before applying it to the HiTrap™ Column column.