Contrast-induced acute kidney injury (CI-AKI) is an iatrogenic complication frequently developed after cardiac catheterization procedures with the administration of iodinatedcontrast media. However, the precise pathological mechanisms remain obscure. Thus, the establishment of a reproducible rat model following intra-arterial contrast medium (CM) injection to simulate the process of arteriography is of great importance. Here, a detailed protocol for establishing a CI-AKI model was describe. Three-month-old male Sprague-Dawley rats, weighing approximately 300~400g at the start of the experiment, were used. The rats were pretreated with water dehydration for 48 hours, plus indomethacin and N-\u03c9 nitro-L-arginine methyl ester (L-NAME) injection, before intra-arterial contrast medium (CM) administration. Levels of serum creatinine (SCr) were measured and histopathological assessment of the kidney tissue by H&E staining were conducted. As expected, the administration of CM, following the pretreatment by indomethacin and L-NAME, induced remarkable renal dysfunction at 12hours post-procedure in comparing with controls. The pronounced histopathological alterations in the tubular epithelial cells of the inner medulla and outer medulla were also observed in the CI-AKI rats. Thus, this rat model was reliable as a valuable tool for exploring the pathogenesis of CI-AKI.