Mar 06, 2024

A RAB7A Phosphoswitch Coordinates Rubicon Homology Protein Regulation of PINK1/Parkin-Dependent Mitophagy - protocols

DOI
https://dx.doi.org/10.17504/protocols.io.3byl4qy7jvo5/v1
  • 1University of California, Berkeley
Dan Tudorica
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DOI: https://dx.doi.org/10.17504/protocols.io.3byl4qy7jvo5/v1
Collection Citation: Dan Tudorica 2024. A RAB7A Phosphoswitch Coordinates Rubicon Homology Protein Regulation of PINK1/Parkin-Dependent Mitophagy - protocols. protocols.io https://dx.doi.org/10.17504/protocols.io.3byl4qy7jvo5/v1
License: This is an open access  collection  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: January 10, 2024
Last Modified: March 06, 2024
Collection  Integer ID: 93365
Keywords: rab7a phosphoswitch coordinates rubicon homology protein regulation, dependent mitophagy, mitophagy, protein, parkin
Abstract
Methods used in this paper.
Files
Protocol
Name
HaloTag autophagy flux assay
Version 1
Image of Dan Tudorica, Hurley Lab, QB3, UC Berkeley
Dan TudoricaHurley Lab, QB3, UC Berkeley
Protocol
Name
Pulldowns
Version 1
Image of Dan Tudorica, Hurley Lab, QB3, UC Berkeley
Dan TudoricaHurley Lab, QB3, UC Berkeley
Protocol
Name
Stable cell line generation via retrovirus
Version 1
Image of Dan Tudorica, Hurley Lab, QB3, UC Berkeley
Dan TudoricaHurley Lab, QB3, UC Berkeley
Protocol
Name
Plasmid construction
Version 1
Image of Dan Tudorica, Hurley Lab, QB3, UC Berkeley
Dan TudoricaHurley Lab, QB3, UC Berkeley
Protocol
Name
Isothermal Titration Calorimetry of the Rubicon RH domain and Rab7
Version 1
Image of Dan Tudorica, Hurley Lab, QB3, UC Berkeley
Dan TudoricaHurley Lab, QB3, UC Berkeley
Protocol
Name
Immunofluorescent Imaging and Analysis
Version 1
Image of Dan Tudorica, Hurley Lab, QB3, UC Berkeley
Dan TudoricaHurley Lab, QB3, UC Berkeley
Protocol
Name
WIPI2d puncta formation assay
Version 1
Image of Dan Tudorica, Hurley Lab, QB3, UC Berkeley
Dan TudoricaHurley Lab, QB3, UC Berkeley
Protocol
Name
Homology Modelling of Pacer RH domain in SWISS-MODEL
Version 1
Image of Dan Tudorica, Hurley Lab, QB3, UC Berkeley
Dan TudoricaHurley Lab, QB3, UC Berkeley
Protocol
Name
Expression and Purification of RH proteins via MBP affinity tag
Version 1
Image of Dan Tudorica, Hurley Lab, QB3, UC Berkeley
Dan TudoricaHurley Lab, QB3, UC Berkeley
Protocol
Name
Confocal-based bead-binding
Version 1
Image of Dan Tudorica, Hurley Lab, QB3, UC Berkeley
Dan TudoricaHurley Lab, QB3, UC Berkeley
Protocol
Name
Rab7 Phosphorylation reaction
Version 1
Image of Dan Tudorica, Hurley Lab, QB3, UC Berkeley
Dan TudoricaHurley Lab, QB3, UC Berkeley
Protocol
Name
Tissue culture of HeLa cells
Version 1
Image of Dan Tudorica, Hurley Lab, QB3, UC Berkeley
Dan TudoricaHurley Lab, QB3, UC Berkeley
Protocol
Name
Western blotting for Rubicon and Pacer expression
Version 1
Image of Dan Tudorica, Hurley Lab, QB3, UC Berkeley
Dan TudoricaHurley Lab, QB3, UC Berkeley